Any feedback?
Literature summary for 1.14.13.25 extracted from
- Hydroxylation of methane through component interactions in soluble methane monooxygenases (2016), J. Microbiol., 54, 277-282 .
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Methylococcus capsulatus | 5737 | - |
| cytoplasm | - |
Methylosinus trichosporium | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | the enzyme has diiron (FeIII-FeIII) active sites where different types of hydrocarbons are oxidized through orchestrated hydroxylase, regulatory and reductase components for precise control of hydrocarbons, oxygen, protons, and electrons | Methylococcus capsulatus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 251000 | - |
- |
Methylococcus capsulatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methane + NAD(P)H + H+ + O2 | Methylococcus capsulatus | methane hydroxylation through methane monooxygenases is a key aspect due to their control of the carbon cycle in the ecology system | methanol + NAD(P)+ + H2O | - |
? | |
| methane + NAD(P)H + H+ + O2 | Methylosinus trichosporium | methane hydroxylation through methane monooxygenases is a key aspect due to their control of the carbon cycle in the ecology system | methanol + NAD(P)+ + H2O | - |
? | |
| methane + NAD(P)H + H+ + O2 | Methylococcus capsulatus Bath. | methane hydroxylation through methane monooxygenases is a key aspect due to their control of the carbon cycle in the ecology system | methanol + NAD(P)+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylococcus capsulatus | P22869 and P18798 and P11987 and P18797 and P22868 and P22867 | P22869 (MmoX), P18798 (MmoY), P11987 (MmoZ), P18797 (MmoB), P22868 (MmoC), P22867 (MmoD). The soluble methane monooxygenase (sMMO) consists of four components A/MMOH (composed of alpha/MmoX, beta/MmoY and gamma/MmoZ), B/MMOB (MmoB), C/MMOR (MmoC) and D/MMOD (MmoD) | - |
| Methylococcus capsulatus Bath. | P22869 and P18798 and P11987 and P18797 and P22868 and P22867 | P22869 (MmoX), P18798 (MmoY), P11987 (MmoZ), P18797 (MmoB), P22868 (MmoC), P22867 (MmoD). The soluble methane monooxygenase (sMMO) consists of four components A/MMOH (composed of alpha/MmoX, beta/MmoY and gamma/MmoZ), B/MMOB (MmoB), C/MMOR (MmoC) and D/MMOD (MmoD) | - |
| Methylosinus trichosporium | P27353 and P27355 and P27354 and P27356 and Q53563 and Q53562 | P27353 (alpha/MmoX), P27355 (gamma/MmoZ), P27354 (beta/MmoY), P27356 (MmoB), Q53563 (MmoC), Q53562 (MmoD). The soluble methane monooxygenase (sMMO) consists of four components A/MMOH (composed of alpha/MmoX, beta/MmoY and gamma/MmoZ), B/MMOB (MmoB), C/MMOR (MmoC) and D/MMOD (MmoD) | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methane + NAD(P)H + H+ + O2 | - |
Methylococcus capsulatus | methanol + NAD(P)+ + H2O | - |
? | |
| methane + NAD(P)H + H+ + O2 | - |
Methylosinus trichosporium | methanol + NAD(P)+ + H2O | - |
? | |
| methane + NAD(P)H + H+ + O2 | methane hydroxylation through methane monooxygenases is a key aspect due to their control of the carbon cycle in the ecology system | Methylococcus capsulatus | methanol + NAD(P)+ + H2O | - |
? | |
| methane + NAD(P)H + H+ + O2 | methane hydroxylation through methane monooxygenases is a key aspect due to their control of the carbon cycle in the ecology system | Methylosinus trichosporium | methanol + NAD(P)+ + H2O | - |
? | |
| methane + NAD(P)H + H+ + O2 | - |
Methylococcus capsulatus Bath. | methanol + NAD(P)+ + H2O | - |
? | |
| methane + NAD(P)H + H+ + O2 | methane hydroxylation through methane monooxygenases is a key aspect due to their control of the carbon cycle in the ecology system | Methylococcus capsulatus Bath. | methanol + NAD(P)+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | the hydroxylase (MMOH) component is a homodimer that consists of two protomers, and each protomer has three polypeptides (alphabetagamma), 60600 (alpha/MmoX) + 40500 (beta/MmoY) + 19800 (gamma/MmoZ), two equivalent of the regulatory component (MmoB) bind to one equivalent MMOH (alpha2beta2gamma2), + reductase protein (MmoC), + 12000 (MmoD) | Methylococcus capsulatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sMMO | - |
Methylococcus capsulatus |
| sMMO | - |
Methylosinus trichosporium |
| soluble methane monooxygenase | - |
Methylosinus trichosporium |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Methylococcus capsulatus | expressed in copper-limited conditions | up |
| Methylosinus trichosporium | expressed in copper-limited conditions | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | methane hydroxylation through methane monooxygenases is a key aspect due to their control of the carbon cycle in the ecology system | Methylococcus capsulatus |
| metabolism | methane hydroxylation through methane monooxygenases is a key aspect due to their control of the carbon cycle in the ecology system | Methylosinus trichosporium |
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