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Literature summary for 1.14.13.25 extracted from
- Identification and characterization of a novel class of self-sufficient cytochrome P450 hydroxylase involved in cyclohexanecarboxylate degradation in Paraburkholderia terrae strain KU-64 (2022), Biosci. Biotechnol. Biochem., 86, 199-208 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Paraburkholderia terrae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
NADH | pH 7.4, temperature not specified in the publication | Paraburkholderia terrae |  |
| 0.015 | - |
cycloheptanecarboxylate | pH 7.4, temperature not specified in the publication | Paraburkholderia terrae | |
| 0.084 | - |
cyclohexanecarboxylate | pH 7.4, temperature not specified in the publication | Paraburkholderia terrae | |
| 0.21 | - |
NADPH | pH 7.4, temperature not specified in the publication | Paraburkholderia terrae | |
| 0.27 | - |
Heptanoate | pH 7.4, temperature not specified in the publication | Paraburkholderia terrae | |
| 0.33 | - |
cyclopentanecarboxylate | pH 7.4, temperature not specified in the publication | Paraburkholderia terrae | |
| 0.51 | - |
hexanoate | pH 7.4, temperature not specified in the publication | Paraburkholderia terrae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paraburkholderia terrae | - |
- |
- |
| Paraburkholderia terrae KU-64 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.37 | - |
pH 7.4, temperature not specified in the publication | Paraburkholderia terrae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cycloheptanecarboxylate + NADH + H+ + O2 | - |
Paraburkholderia terrae | trans-4-hydroxycycloheptane-1-carboxylate + NAD+ + H2O | - |
? | |
| cycloheptanecarboxylate + NADH + H+ + O2 | - |
Paraburkholderia terrae KU-64 | trans-4-hydroxycycloheptane-1-carboxylate + NAD+ + H2O | - |
? | |
| cyclohexanecarboxylate + NADH + H+ + O2 | - |
Paraburkholderia terrae | trans-4-hydroxycyclohexane-1-carboxylate + NAD+ + H2O | - |
? | |
| cyclohexanecarboxylate + NADH + H+ + O2 | - |
Paraburkholderia terrae KU-64 | trans-4-hydroxycyclohexane-1-carboxylate + NAD+ + H2O | - |
? | |
| cyclopentanecarboxylate + NADH + H+ + O2 | - |
Paraburkholderia terrae | trans-3-hydroxycyclopentane-1-carboxylate + NAD+ + H2O | - |
? | |
| cyclopentanecarboxylate + NADH + H+ + O2 | - |
Paraburkholderia terrae KU-64 | trans-3-hydroxycyclopentane-1-carboxylate + NAD+ + H2O | - |
? | |
| heptanoate + NADPH + H+ + O2 | - |
Paraburkholderia terrae | ? + NADP+ + H2O | - |
? | |
| heptanoate + NADPH + H+ + O2 | - |
Paraburkholderia terrae KU-64 | ? + NADP+ + H2O | - |
? | |
| hexanoate + NADH + H+ + O2 | - |
Paraburkholderia terrae | ? + NAD+ + H2O | - |
? | |
| hexanoate + NADH + H+ + O2 | - |
Paraburkholderia terrae KU-64 | ? + NAD+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | protein comprises a [2Fe-2S] ferredoxin domain, NAD(P)H-dependent FAD-containing reductase domain, FCD domain, and cytochrome P450 domain, in that order from the N terminus | Paraburkholderia terrae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chcA | - |
Paraburkholderia terrae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
Heptanoate | pH 7.4, temperature not specified in the publication | Paraburkholderia terrae | |
| 3.5 | - |
hexanoate | pH 7.4, temperature not specified in the publication | Paraburkholderia terrae | |
| 4.8 | - |
cycloheptanecarboxylate | pH 7.4, temperature not specified in the publication | Paraburkholderia terrae | |
| 5.6 | - |
cyclopentanecarboxylate | pH 7.4, temperature not specified in the publication | Paraburkholderia terrae | |
| 15.9 | - |
cyclohexanecarboxylate | pH 7.4, temperature not specified in the publication | Paraburkholderia terrae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Paraburkholderia terrae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Paraburkholderia terrae | |
| Ferredoxin | - |
Paraburkholderia terrae | |
| NADH | NADH is largely preferred over NAPDH | Paraburkholderia terrae | |
| NADPH | NADH is largely preferred over NAPDH | Paraburkholderia terrae | |
| [2Fe-2S]-center | - |
Paraburkholderia terrae | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | enzyme is a self-sufficient cytochrome P450 | Paraburkholderia terrae |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.5 | - |
Heptanoate | pH 7.4, temperature not specified in the publication | Paraburkholderia terrae | |
| 6.9 | - |
hexanoate | pH 7.4, temperature not specified in the publication | Paraburkholderia terrae | |
| 17.3 | - |
cyclopentanecarboxylate | pH 7.4, temperature not specified in the publication | Paraburkholderia terrae | |
| 189 | - |
cyclohexanecarboxylate | pH 7.4, temperature not specified in the publication | Paraburkholderia terrae | |
| 331 | - |
cycloheptanecarboxylate | pH 7.4, temperature not specified in the publication | Paraburkholderia terrae |
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