BRENDA - Enzyme Database
show all sequences of 1.14.13.27

Purification and properties of 4-aminobenzoate hydroxylase, a new monooxygenase from Agaricus bisporus

Tsuji, H.; Ogawa, T.; Bando, N.; Sasaoka, K.; J. Biol. Chem. 261, 13203-13209 (1986)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
AgNO3
93% inactivation at 0.1 mM
Agaricus bisporus
Br-
37% inactivation at 0.1 M
Agaricus bisporus
Cl-
30% inactivation at 0.1 M
Agaricus bisporus
CuSO4
91% inactivation at 0.1 mM
Agaricus bisporus
F-
20% inactivation at 0.1 M
Agaricus bisporus
HgCl2
92% inactivation at 0.1 mM
Agaricus bisporus
I-
62% inactivation at 0.1 M
Agaricus bisporus
NO3-
45% inactivation at 0.1 M
Agaricus bisporus
p-chloromercuribenzoate
94% inactivation at 0.1 mM
Agaricus bisporus
SCN-
60% inactivation at 0.1 M
Agaricus bisporus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0136
-
NADH
-
Agaricus bisporus
0.0204
-
4-Aminobenzoate
-
Agaricus bisporus
0.133
-
NADPH
-
Agaricus bisporus
0.2
-
O2
-
Agaricus bisporus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49000
-
gel filtration
Agaricus bisporus
50000
-
SDS-PAGE
Agaricus bisporus
Organism
Organism
UniProt
Commentary
Textmining
Agaricus bisporus
-
mushroom
-
Purification (Commentary)
Purification (Commentary)
Organism
homogeneity
Agaricus bisporus
Storage Stability
Storage Stability
Organism
-20C, 50 mM potassium phosphate buffer, pH 7.0, 20% glycerol, 1 mM 2-mercaptoethanol, 0.01 mM FAD, stable for 1 month
Agaricus bisporus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-amino-5-chlorobenzoate + NAD(P)H + O2
-
438909
Agaricus bisporus
4-chloro-2-hydroxyaniline + NAD(P)+ + H2O2 + CO2
H2O2 formed simultaneously with hydroxylation
438909
Agaricus bisporus
?
3,4-diaminobenzoate + NAD(P)H + O2
17.3% of the reaction with 4-aminobenzoate
438909
Agaricus bisporus
2-amino-4-hydroxyaniline + NAD(P)+ + H2O2 + CO2
H2O2 formed simultaneously with hydroxylation
438909
Agaricus bisporus
?
4-amino-2-chlorobenzoate + NAD(P)H + O2
-
438909
Agaricus bisporus
3-chloro-4-hydroxyaniline + NAD(P)+ + H2O2 + CO2
H2O2 formed simultaneously with hydroxylation
438909
Agaricus bisporus
?
4-aminobenzoate + NAD(P)H + O2
-
438909
Agaricus bisporus
4-hydroxyaniline + NAD(P)+ + H2O + CO2
-
438909
Agaricus bisporus
?
4-aminosalicylate + NAD(P)H + O2
-
438909
Agaricus bisporus
3,4-dihydroxyaniline + NAD(P)+ + H2O2 + CO2
H2O2 formed simultaneously with hydroxylation
438909
Agaricus bisporus
?
4-hydroxybenzoate + NAD(P)H + O2
8.4% of the reaction with 4-aminobenzoate
438909
Agaricus bisporus
quinol + NAD(P)+ + H2O2 + CO2
H2O2 formed simultaneously with hydroxylation
438909
Agaricus bisporus
?
anthranilate + NAD(P)H + O2
-
438909
Agaricus bisporus
2-hydroxyaniline + NAD(P)+ + H2O2 + CO2
H2O2 formed simultaneously with hydroxylation
438909
Agaricus bisporus
?
additional information
several aromatic compounds stimulate NADH oxidation, but no hydroxylated product is formed, all of the O2 consumed is converted to H2O2
438909
Agaricus bisporus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 49000, SDS-PAGE
Agaricus bisporus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
-
Agaricus bisporus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
-
60 min, little loss of activity in presence of 0.1 mM FAD and 0.02% bovine serum albumin
Agaricus bisporus
35
-
10 min, 50 mM potassium phosphate buffer, pH 7.0, 0.1 mM FAD, 0.02% bovine serum albumin, stable
Agaricus bisporus
40
-
10 min, 50 mM potassium phosphate buffer, pH 7.0, 0.1 mM FAD, 0.02% bovine serum albumin, about 70% loss of activity
Agaricus bisporus
45
-
10 min, 50 mM potassium phosphate buffer, pH 7.0, 0.1 mM FAD, 0.02% bovine serum albumin, 97% loss of activity
Agaricus bisporus
50
-
10 min, 50 mM potassium phosphate buffer, pH 7.0, 0.1 mM FAD, 0.02% bovine serum albumin, 98% loss of activity
Agaricus bisporus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
NADPH
Agaricus bisporus
6.5
8
NADH
Agaricus bisporus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
8
stable
Agaricus bisporus
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
0.91 mol of FAD per mol of enzyme; flavoprotein
Agaricus bisporus
NADH
-
Agaricus bisporus
NADPH
62% of the activity with NADH; 68% of the activity with NADH
Agaricus bisporus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
0.91 mol of FAD per mol of enzyme; flavoprotein
Agaricus bisporus
NADH
-
Agaricus bisporus
NADPH
62% of the activity with NADH; 68% of the activity with NADH
Agaricus bisporus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
AgNO3
93% inactivation at 0.1 mM
Agaricus bisporus
Br-
37% inactivation at 0.1 M
Agaricus bisporus
Cl-
30% inactivation at 0.1 M
Agaricus bisporus
CuSO4
91% inactivation at 0.1 mM
Agaricus bisporus
F-
20% inactivation at 0.1 M
Agaricus bisporus
HgCl2
92% inactivation at 0.1 mM
Agaricus bisporus
I-
62% inactivation at 0.1 M
Agaricus bisporus
NO3-
45% inactivation at 0.1 M
Agaricus bisporus
p-chloromercuribenzoate
94% inactivation at 0.1 mM
Agaricus bisporus
SCN-
60% inactivation at 0.1 M
Agaricus bisporus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0136
-
NADH
-
Agaricus bisporus
0.0204
-
4-Aminobenzoate
-
Agaricus bisporus
0.133
-
NADPH
-
Agaricus bisporus
0.2
-
O2
-
Agaricus bisporus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49000
-
gel filtration
Agaricus bisporus
50000
-
SDS-PAGE
Agaricus bisporus
Purification (Commentary) (protein specific)
Commentary
Organism
homogeneity
Agaricus bisporus
Storage Stability (protein specific)
Storage Stability
Organism
-20C, 50 mM potassium phosphate buffer, pH 7.0, 20% glycerol, 1 mM 2-mercaptoethanol, 0.01 mM FAD, stable for 1 month
Agaricus bisporus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-amino-5-chlorobenzoate + NAD(P)H + O2
-
438909
Agaricus bisporus
4-chloro-2-hydroxyaniline + NAD(P)+ + H2O2 + CO2
H2O2 formed simultaneously with hydroxylation
438909
Agaricus bisporus
?
3,4-diaminobenzoate + NAD(P)H + O2
17.3% of the reaction with 4-aminobenzoate
438909
Agaricus bisporus
2-amino-4-hydroxyaniline + NAD(P)+ + H2O2 + CO2
H2O2 formed simultaneously with hydroxylation
438909
Agaricus bisporus
?
4-amino-2-chlorobenzoate + NAD(P)H + O2
-
438909
Agaricus bisporus
3-chloro-4-hydroxyaniline + NAD(P)+ + H2O2 + CO2
H2O2 formed simultaneously with hydroxylation
438909
Agaricus bisporus
?
4-aminobenzoate + NAD(P)H + O2
-
438909
Agaricus bisporus
4-hydroxyaniline + NAD(P)+ + H2O + CO2
-
438909
Agaricus bisporus
?
4-aminosalicylate + NAD(P)H + O2
-
438909
Agaricus bisporus
3,4-dihydroxyaniline + NAD(P)+ + H2O2 + CO2
H2O2 formed simultaneously with hydroxylation
438909
Agaricus bisporus
?
4-hydroxybenzoate + NAD(P)H + O2
8.4% of the reaction with 4-aminobenzoate
438909
Agaricus bisporus
quinol + NAD(P)+ + H2O2 + CO2
H2O2 formed simultaneously with hydroxylation
438909
Agaricus bisporus
?
anthranilate + NAD(P)H + O2
-
438909
Agaricus bisporus
2-hydroxyaniline + NAD(P)+ + H2O2 + CO2
H2O2 formed simultaneously with hydroxylation
438909
Agaricus bisporus
?
additional information
several aromatic compounds stimulate NADH oxidation, but no hydroxylated product is formed, all of the O2 consumed is converted to H2O2
438909
Agaricus bisporus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 49000, SDS-PAGE
Agaricus bisporus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
-
Agaricus bisporus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
-
60 min, little loss of activity in presence of 0.1 mM FAD and 0.02% bovine serum albumin
Agaricus bisporus
35
-
10 min, 50 mM potassium phosphate buffer, pH 7.0, 0.1 mM FAD, 0.02% bovine serum albumin, stable
Agaricus bisporus
40
-
10 min, 50 mM potassium phosphate buffer, pH 7.0, 0.1 mM FAD, 0.02% bovine serum albumin, about 70% loss of activity
Agaricus bisporus
45
-
10 min, 50 mM potassium phosphate buffer, pH 7.0, 0.1 mM FAD, 0.02% bovine serum albumin, 97% loss of activity
Agaricus bisporus
50
-
10 min, 50 mM potassium phosphate buffer, pH 7.0, 0.1 mM FAD, 0.02% bovine serum albumin, 98% loss of activity
Agaricus bisporus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
NADPH
Agaricus bisporus
6.5
8
NADH
Agaricus bisporus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
8
stable
Agaricus bisporus
Other publictions for EC 1.14.13.27
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
438912
Mizutani
A new UV method for serum gamm ...
Agaricus bisporus
J. Biochem.
126
347-353
1999
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-
1
-
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1
2
-
-
-
-
-
3
-
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1
-
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-
1
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2
-
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-
-
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-
1
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-
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1
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1
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2
-
-
-
-
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-
-
1
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
438913
Tsuji
Epitope mapping of monoclonal ...
Agaricus bisporus
Biochim. Biophys. Acta
1425
628-631
1998
-
-
1
-
-
-
-
-
-
-
-
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-
2
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
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-
1
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-
1
1
-
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-
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-
-
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-
-
-
-
-
-
-
-
-
-
-
-
438914
Tsuji
Cloning and sequencing of cDNA ...
Agaricus bisporus
Biochim. Biophys. Acta
1309
31-36
1996
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
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1
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1
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-
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-
-
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-
-
-
-
-
-
-
-
438910
Tsuji
-
Stereospecificity of NAD(P)H o ...
Agaricus bisporus
Biochim. Biophys. Acta
991
380-382
1989
-
-
-
-
-
-
-
-
-
-
-
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-
1
-
-
1
1
-
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1
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1
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1
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1
-
1
-
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-
-
-
-
-
-
-
-
-
-
438909
Tsuji
Purification and properties of ...
Agaricus bisporus
J. Biol. Chem.
261
13203-13209
1986
-
-
-
-
-
-
10
4
-
-
2
-
-
2
-
-
1
-
-
-
-
1
8
1
-
1
-
5
-
2
-
1
3
-
-
-
-
-
-
3
-
-
-
-
10
-
4
-
-
2
-
-
-
-
1
-
-
-
1
8
1
1
-
5
-
2
-
1
-
-
-
-
-
-
-
438911
Tsuji
A unique enzyme catalyzing the ...
Agaricus bisporus
Biochem. Biophys. Res. Commun.
130
633-639
1985
-
-
-
-
-
-
4
-
-
-
-
-
-
1
-
-
1
-
-
1
1
1
7
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
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-
4
-
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-
1
-
1
1
1
7
-
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-
-
-
1
-
-
-
-
-
-
-
-
-