Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas nitroreducens |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour-diffusion, optimized precipitant solution contains 1.6 M ammonium sulfate, 100 mM sodium chloride and 100 mM MES-NaOH, pH 7.5, crystals of native and SeMet-HbpA diffract to 2.01 and 2.25 A, respectively | Pseudomonas nitroreducens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
mercury | - |
Pseudomonas nitroreducens | |
platinum | - |
Pseudomonas nitroreducens | |
silver | - |
Pseudomonas nitroreducens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
256000 | - |
- |
Pseudomonas nitroreducens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxybiphenyl + NADH + O2 | Pseudomonas nitroreducens | first enzyme of the 2-hydroxybiphenyl degradation pathway | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas nitroreducens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant HbpA | Pseudomonas nitroreducens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxybiphenyl + NADH + O2 | - |
Pseudomonas nitroreducens | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? | |
2-hydroxybiphenyl + NADH + O2 | first enzyme of the 2-hydroxybiphenyl degradation pathway | Pseudomonas nitroreducens | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Pseudomonas nitroreducens |
Synonyms | Comment | Organism |
---|---|---|
HbpA | - |
Pseudomonas nitroreducens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | each subunit contains a noncovalently but tightly bound FAD | Pseudomonas nitroreducens | |
NADH | - |
Pseudomonas nitroreducens |