BRENDA - Enzyme Database show
show all sequences of 1.14.14.102

Systematic silencing of benzylisoquinoline alkaloid biosynthetic genes reveals the major route to papaverine in opium poppy

Desgagne-Penix, I.; Facchini, P.; Plant J. 72, 331-344 (2012)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
Papaver somniferum
-
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
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-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Papaver somniferum
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
-
720710
Papaver somniferum
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
Papaver somniferum
-
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
-
720710
Papaver somniferum
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
enzyme silencing is associated with a 22% reduction in total alkaloid accumulation, with a notable decrease in the levels of thebaine and codeine compared with controls. In contrast, absolute levels of morphine and papaverine are not affected
Papaver somniferum
General Information (protein specific)
General Information
Commentary
Organism
malfunction
enzyme silencing is associated with a 22% reduction in total alkaloid accumulation, with a notable decrease in the levels of thebaine and codeine compared with controls. In contrast, absolute levels of morphine and papaverine are not affected
Papaver somniferum
Other publictions for EC 1.14.14.102
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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720710
Desgagne-Penix
Systematic silencing of benzyl ...
Papaver somniferum
Plant J.
72
331-344
2012
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701436
Zhou
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Molecular cloning and sequence ...
Coptis chinensis
Zhong Cao Yao
39
419-423
2008
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688821
Frick
Metabolic engineering with a m ...
Papaver somniferum
Metab. Eng.
9
169-176
2007
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670561
Samanani
The role of phloem sieve eleme ...
Papaver somniferum
Plant J.
47
547-563
2006
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660256
Alcantara
Sanguinarine biosynthesis is a ...
Papaver somniferum
Plant Physiol.
138
173-183
2005
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660086
Huang
Distribution of morphinan and ...
Papaver bracteatum, Papaver orientale, Papaver rhoeas, Papaver somniferum
Phytochemistry
53
555-564
2000
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285356
Pauli
Molecular cloning and function ...
Eschscholzia californica
Plant J.
13
793-801
1998
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