BRENDA - Enzyme Database show
show all sequences of 1.14.14.103

A cytochrome P-450 monooxygenase catalyzes the first step in the conversion of tabersonine to vindoline in Catharanthus roseus

St-Pierre, B.; De Luca, V.; Plant Physiol. 109, 131-139 (1995)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
clotrimazole
50% inhibition at 0.05 mM
Catharanthus roseus
CO
concentration dependent competitive inhibition, 77% inhibition at 90%
Catharanthus roseus
cytochrome c
50% inhibition at 0.001 mM
Catharanthus roseus
miconazole
50% inhibition at 0.3 mM
Catharanthus roseus
tabersonine
inhibition above 0.03 mM
Catharanthus roseus
tricliphane
50% inhibition at 0.5 mM
Catharanthus roseus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.011
-
tabersonine
-
Catharanthus roseus
0.014
-
NADPH
-
Catharanthus roseus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Catharanthus roseus
5783
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tabersonine + NADPH + H+ + O2
Catharanthus roseus
first step in vindoline boxynthesis
16-hydroxytabersonine + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Catharanthus roseus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
bud
-
Catharanthus roseus
-
leaf
-
Catharanthus roseus
-
root
-
Catharanthus roseus
-
seedling
-
Catharanthus roseus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
tabersonine + NADPH + H+ + O2
NADH is less active as substrate
438776
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
438776
Catharanthus roseus
?
tabersonine + NADPH + H+ + O2
first step in vindoline boxynthesis
438776
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Catharanthus roseus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
phosphate buffer
Catharanthus roseus
8
-
Tris buffer
Catharanthus roseus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
clotrimazole
50% inhibition at 0.05 mM
Catharanthus roseus
CO
concentration dependent competitive inhibition, 77% inhibition at 90%
Catharanthus roseus
cytochrome c
50% inhibition at 0.001 mM
Catharanthus roseus
miconazole
50% inhibition at 0.3 mM
Catharanthus roseus
tabersonine
inhibition above 0.03 mM
Catharanthus roseus
tricliphane
50% inhibition at 0.5 mM
Catharanthus roseus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.011
-
tabersonine
-
Catharanthus roseus
0.014
-
NADPH
-
Catharanthus roseus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Catharanthus roseus
5783
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tabersonine + NADPH + H+ + O2
Catharanthus roseus
first step in vindoline boxynthesis
16-hydroxytabersonine + NADP+ + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
bud
-
Catharanthus roseus
-
leaf
-
Catharanthus roseus
-
root
-
Catharanthus roseus
-
seedling
-
Catharanthus roseus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
tabersonine + NADPH + H+ + O2
NADH is less active as substrate
438776
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
438776
Catharanthus roseus
?
tabersonine + NADPH + H+ + O2
first step in vindoline boxynthesis
438776
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Catharanthus roseus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
phosphate buffer
Catharanthus roseus
8
-
Tris buffer
Catharanthus roseus
Other publictions for EC 1.14.14.103
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743890
Liu
Enhancement of vindoline and ...
Catharanthus roseus
World J. Microbiol. Biotechnol.
30
175-180
2014
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1
1
1
1
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728525
Besseau
A pair of tabersonine 16-hydro ...
Catharanthus roseus
Plant Physiol.
163
1792-1803
2013
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1
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1
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2
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4
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1
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712866
Guirimand
Spatial organization of the vi ...
Catharanthus roseus
J. Plant Physiol.
168
549-557
2010
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1
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1
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1
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1
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1
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676372
Magnotta
Identification of a low vindol ...
Catharanthus roseus
Phytochemistry
67
1758-1764
2006
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1
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670558
Murata
localization of tabersonine 16 ...
Catharanthus roseus
Plant J.
44
581-594
2005
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1
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1
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438775
Schroder
Light-induced cytochrome P450- ...
Catharanthus roseus
FEBS Lett.
458
97-102
1999
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438776
St-Pierre
A cytochrome P-450 monooxygena ...
Catharanthus roseus
Plant Physiol.
109
131-139
1995
-
-
-
-
-
-
6
2
1
-
-
1
-
3
-
-
-
-
-
4
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2
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1
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2
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6
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1
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