BRENDA - Enzyme Database show
show all sequences of 1.14.14.103

A pair of tabersonine 16-hydroxylases initiates the synthesis of vindoline in an organ-dependent manner in Catharanthus roseus

Besseau, S.; Kellner, F.; Lanoue, A.; Thamm, A.M.; Salim, V.; Schneider, B.; Geu-Flores, F.; Hoefer, R.; Guirimand, G.; Guihur, A.; Oudin, A.; Glevarec, G.; Foureau, E.; Papon, N.; Clastre, M.; Giglioli-Guivarch, N.; St-Pierre, B.; Werck-Reichhart, D.; Burlat, V.; De Luca, V.; OConnor, S.E.; Cou, C.o.u.r.; Plant Physiol. 163, 1792-1803 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Saccharomyces cerevisiae WAT11 cells
Catharanthus roseus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00007
-
tabersonine
isoform T16H2, at pH 8.0 and 30°C
Catharanthus roseus
0.00035
-
tabersonine
isoform T16H1, at pH 8.0 and 30°C
Catharanthus roseus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
CYP71D351 (isoform T16H2)
Catharanthus roseus
5783
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2,3-dihydro-3-hydroxytabersonine + [reduced NADPH-hemoprotein reductase] + O2
Catharanthus roseus
low activity
2,3-dihydro-3,16-dihydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
2,3-dihydrotabersonine + [reduced NADPH-hemoprotein reductase] + O2
Catharanthus roseus
low activity
2,3-dihydro-16-hydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
additional information
Catharanthus roseus
no activity with naringenin, tryptamine, secologanin, strictosidine, ajmalicine, vindoline, and catharanthine
?
-
-
-
tabersonine + NADPH + H+ + O2
Catharanthus roseus
highest activity
16-hydroxytabersonine + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Catharanthus roseus
C0KYN4
CYP71D12; cultivar Pacifica Pink
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
isoform T16H1, CYP71D351
Catharanthus roseus
-
leaf epidermis
highest expression of isoform T16H2, CYP71D12 in young leaves
Catharanthus roseus
-
additional information
CYP71D12 transcripts are barely detectable or undetectable in roots, internodes, young and mature leaves, and flower buds; CYP71D351 transcripts are barely detectable or undetectable in roots, internodes, young and mature leaves, and flower buds
Catharanthus roseus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-dihydro-3-hydroxytabersonine + [reduced NADPH-hemoprotein reductase] + O2
low activity
728525
Catharanthus roseus
2,3-dihydro-3,16-dihydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
2,3-dihydrotabersonine + [reduced NADPH-hemoprotein reductase] + O2
low activity
728525
Catharanthus roseus
2,3-dihydro-16-hydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
no activity with naringenin, tryptamine, secologanin, strictosidine, ajmalicine, vindoline, and catharanthine
728525
Catharanthus roseus
?
-
-
-
-
tabersonine + NADPH + H+ + O2
highest activity
728525
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Saccharomyces cerevisiae WAT11 cells
Catharanthus roseus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00007
-
tabersonine
isoform T16H2, at pH 8.0 and 30°C
Catharanthus roseus
0.00035
-
tabersonine
isoform T16H1, at pH 8.0 and 30°C
Catharanthus roseus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
CYP71D351 (isoform T16H2)
Catharanthus roseus
5783
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2,3-dihydro-3-hydroxytabersonine + [reduced NADPH-hemoprotein reductase] + O2
Catharanthus roseus
low activity
2,3-dihydro-3,16-dihydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
2,3-dihydrotabersonine + [reduced NADPH-hemoprotein reductase] + O2
Catharanthus roseus
low activity
2,3-dihydro-16-hydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
additional information
Catharanthus roseus
no activity with naringenin, tryptamine, secologanin, strictosidine, ajmalicine, vindoline, and catharanthine
?
-
-
-
tabersonine + NADPH + H+ + O2
Catharanthus roseus
highest activity
16-hydroxytabersonine + NADP+ + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
isoform T16H1, CYP71D351
Catharanthus roseus
-
leaf epidermis
highest expression of isoform T16H2, CYP71D12 in young leaves
Catharanthus roseus
-
additional information
CYP71D12 transcripts are barely detectable or undetectable in roots, internodes, young and mature leaves, and flower buds; CYP71D351 transcripts are barely detectable or undetectable in roots, internodes, young and mature leaves, and flower buds
Catharanthus roseus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-dihydro-3-hydroxytabersonine + [reduced NADPH-hemoprotein reductase] + O2
low activity
728525
Catharanthus roseus
2,3-dihydro-3,16-dihydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
2,3-dihydrotabersonine + [reduced NADPH-hemoprotein reductase] + O2
low activity
728525
Catharanthus roseus
2,3-dihydro-16-hydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
no activity with naringenin, tryptamine, secologanin, strictosidine, ajmalicine, vindoline, and catharanthine
728525
Catharanthus roseus
?
-
-
-
-
tabersonine + NADPH + H+ + O2
highest activity
728525
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
CYP71D12 (isoform T16H2) plays a role in the biosynthesis of vindoline in flowers; CYP71D351 (isoform T16H2) has a major role in the biosynthesis of vindoline in leaves
Catharanthus roseus
General Information (protein specific)
General Information
Commentary
Organism
metabolism
CYP71D12 (isoform T16H2) plays a role in the biosynthesis of vindoline in flowers; CYP71D351 (isoform T16H2) has a major role in the biosynthesis of vindoline in leaves
Catharanthus roseus
Other publictions for EC 1.14.14.103
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743890
Liu
Enhancement of vindoline and ...
Catharanthus roseus
World J. Microbiol. Biotechnol.
30
175-180
2014
-
1
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2
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2
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2
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1
1
1
1
-
-
728525
Besseau
A pair of tabersonine 16-hydro ...
Catharanthus roseus
Plant Physiol.
163
1792-1803
2013
-
-
1
-
-
-
-
2
1
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4
-
2
-
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3
-
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4
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1
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2
1
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4
-
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3
-
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4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
712866
Guirimand
Spatial organization of the vi ...
Catharanthus roseus
J. Plant Physiol.
168
549-557
2010
-
-
1
-
-
-
-
-
3
-
-
1
-
3
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
676372
Magnotta
Identification of a low vindol ...
Catharanthus roseus
Phytochemistry
67
1758-1764
2006
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
2
-
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3
-
1
-
-
-
1
-
-
1
-
-
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-
-
1
-
-
-
-
-
-
-
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2
-
-
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-
-
2
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
670558
Murata
localization of tabersonine 16 ...
Catharanthus roseus
Plant J.
44
581-594
2005
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1
-
-
-
-
-
-
-
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2
-
3
-
-
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-
-
4
-
-
2
-
1
-
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-
-
-
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1
-
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-
1
1
-
-
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-
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-
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2
-
-
-
-
-
4
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
438775
Schroder
Light-induced cytochrome P450- ...
Catharanthus roseus
FEBS Lett.
458
97-102
1999
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-
1
-
-
-
-
-
-
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1
1
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3
-
-
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2
-
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1
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1
1
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1
1
-
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-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
438776
St-Pierre
A cytochrome P-450 monooxygena ...
Catharanthus roseus
Plant Physiol.
109
131-139
1995
-
-
-
-
-
-
6
2
1
-
-
1
-
3
-
-
-
-
-
4
-
-
2
-
1
-
-
-
2
-
-
-
-
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-
-
-
-
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-
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6
-
2
1
-
-
1
-
-
-
-
-
4
-
-
2
-
1
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-
-
2
-
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