BRENDA - Enzyme Database show
show all sequences of 1.14.14.117

Enzymological evidence for separate pathways for aflatoxin B1 and B2 biosynthesis

Bhatnagar, D.; Biochemistry 30, 4343-4350 (1991)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0012
-
8-O-methylsterigmatocystin
in 50 mM phosphate buffer, pH 7.5, at 29°C
Aspergillus parasiticus
0.0134
-
8-O-methyldihydrosterigmatocystin
in 50 mM phosphate buffer, pH 7.5, at 29°C
Aspergillus parasiticus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus flavus
-
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus
-
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus SRRC 163
-
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus flavus SRRC 141
-
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus flavus
preferred substrate
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus
preferred substrate
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus SRRC 163
preferred substrate
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus flavus SRRC 141
preferred substrate
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus flavus
-
-
-
Aspergillus flavus SRRC 141
-
-
-
Aspergillus parasiticus
-
-
-
Aspergillus parasiticus SRRC 163
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate precipitation, QMA anion exchange column chromatography, and Bio-Gel P-200 gel filtration
Aspergillus flavus
ammonium sulfate precipitation, QMA anion exchange column chromatography, and Bio-Gel P-200 gel filtration
Aspergillus parasiticus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.23
-
enzyme from supernatant, using 8-O-methylsterigmatocystin as substrate, at pH 7.5 and 29°C
Aspergillus parasiticus
34.11
-
after 148.3fold purification, using 8-O-methylsterigmatocystin as substrate, at pH 7.5 and 29°C
Aspergillus parasiticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
721574
Aspergillus flavus
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
721574
Aspergillus parasiticus
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
721574
Aspergillus parasiticus SRRC 163
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
721574
Aspergillus flavus SRRC 141
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
preferred substrate
721574
Aspergillus flavus
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
preferred substrate
721574
Aspergillus parasiticus
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
preferred substrate
721574
Aspergillus parasiticus SRRC 163
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
preferred substrate
721574
Aspergillus flavus SRRC 141
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
30
-
Aspergillus flavus
25
30
-
Aspergillus parasiticus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Aspergillus flavus
7.5
-
-
Aspergillus parasiticus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0012
-
8-O-methylsterigmatocystin
in 50 mM phosphate buffer, pH 7.5, at 29°C
Aspergillus parasiticus
0.0134
-
8-O-methyldihydrosterigmatocystin
in 50 mM phosphate buffer, pH 7.5, at 29°C
Aspergillus parasiticus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus flavus
-
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus
-
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus SRRC 163
-
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus flavus SRRC 141
-
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus flavus
preferred substrate
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus
preferred substrate
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus SRRC 163
preferred substrate
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus flavus SRRC 141
preferred substrate
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, QMA anion exchange column chromatography, and Bio-Gel P-200 gel filtration
Aspergillus flavus
ammonium sulfate precipitation, QMA anion exchange column chromatography, and Bio-Gel P-200 gel filtration
Aspergillus parasiticus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.23
-
enzyme from supernatant, using 8-O-methylsterigmatocystin as substrate, at pH 7.5 and 29°C
Aspergillus parasiticus
34.11
-
after 148.3fold purification, using 8-O-methylsterigmatocystin as substrate, at pH 7.5 and 29°C
Aspergillus parasiticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
721574
Aspergillus flavus
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
721574
Aspergillus parasiticus
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
721574
Aspergillus parasiticus SRRC 163
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
721574
Aspergillus flavus SRRC 141
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
preferred substrate
721574
Aspergillus flavus
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
preferred substrate
721574
Aspergillus parasiticus
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
preferred substrate
721574
Aspergillus parasiticus SRRC 163
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
preferred substrate
721574
Aspergillus flavus SRRC 141
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
30
-
Aspergillus flavus
25
30
-
Aspergillus parasiticus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Aspergillus flavus
7.5
-
-
Aspergillus parasiticus
Other publictions for EC 1.14.14.117
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723750
Jamali
Expression of aflatoxin genes ...
Aspergillus flavus, Aspergillus flavus PFCC 101, Aspergillus flavus PFCC 116, Aspergillus flavus PFCC 137, Aspergillus flavus PFCC 176, Aspergillus flavus PFCC 209, Aspergillus flavus PFCC 247, Aspergillus flavus PFCC 267, Aspergillus flavus PFCC 309, Aspergillus parasiticus, Aspergillus parasiticus NRRL 2999, Aspergillus parasiticus PFCC 14
Res. Microbiol.
164
293-299
2013
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12
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
722440
Udwary
Synthesis of 11-hydroxyl O-met ...
Aspergillus parasiticus, Aspergillus parasiticus SU-1
J. Am. Chem. Soc.
124
5294-5303
2002
-
-
1
-
-
-
-
-
1
-
1
4
-
4
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4
1
-
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1
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1
-
1
4
-
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4
1
-
-
-
-
-
-
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-
-
-
-
-
-
-
721325
Yu
Characterization of the critic ...
Aspergillus parasiticus, Aspergillus parasiticus SRRC 143
Appl. Environ. Microbiol.
64
4834-4841
1998
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-
1
-
3
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4
-
2
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1
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4
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1
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3
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4
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1
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4
-
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1
1
-
-
-
721574
Bhatnagar
Enzymological evidence for sep ...
Aspergillus flavus, Aspergillus flavus SRRC 141, Aspergillus parasiticus, Aspergillus parasiticus SRRC 163
Biochemistry
30
4343-4350
1991
-
-
-
-
-
-
-
2
-
-
-
8
-
4
-
-
2
-
-
-
2
-
8
-
2
-
-
-
2
-
-
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-
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-
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-
-
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-
-
-
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-
-
2
-
-
-
8
-
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2
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2
-
8
-
2
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2
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