BRENDA - Enzyme Database show
show all sequences of 1.14.14.120

The Cyt P450 enzyme CYP716A47 catalyzes the formation of protopanaxadiol from dammarenediol-II during ginsenoside biosynthesis in Panax ginseng

Han, J.Y.; Kim, H.J.; Kwon, Y.S.; Choi, Y.E.; Plant Cell Physiol. 52, 2062-2073 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Saccharomyces cerevisiae strain WAT21
Panax ginseng
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dammarenediol-II + [reduced NADPH-hemoprotein reductase] + O2
Panax ginseng
-
protopanaxadiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Panax ginseng
-
cultivar C.A. Meyer
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
-
Panax ginseng
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dammarenediol-II + [reduced NADPH-hemoprotein reductase] + O2
-
726142
Panax ginseng
protopanaxadiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Saccharomyces cerevisiae strain WAT21
Panax ginseng
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dammarenediol-II + [reduced NADPH-hemoprotein reductase] + O2
Panax ginseng
-
protopanaxadiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
-
Panax ginseng
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dammarenediol-II + [reduced NADPH-hemoprotein reductase] + O2
-
726142
Panax ginseng
protopanaxadiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Expression
Organism
Commentary
Expression
Panax ginseng
the gene is transcriptionally activated not only by methyl jasmonate treatment but also in transgenic ginseng that overexpresses squalene synthase and overproduces ginsenosides
up
Expression (protein specific)
Organism
Commentary
Expression
Panax ginseng
the gene is transcriptionally activated not only by methyl jasmonate treatment but also in transgenic ginseng that overexpresses squalene synthase and overproduces ginsenosides
up
Other publictions for EC 1.14.14.120
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744549
Zhao
Optimization of a cytochrome ...
Panax ginseng
Biotechnol. Bioeng.
113
1787-1795
2016
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-
1
-
1
-
-
-
1
-
-
1
-
5
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
746053
Chun
Production of the dammarene s ...
Panax ginseng
Plant Cell Rep.
34
1551-1560
2015
-
-
1
-
-
-
-
-
1
-
-
1
-
3
-
-
-
-
-
2
-
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1
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2
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1
2
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1
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1
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2
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1
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1
2
2
1
-
-
745011
Wang
The isolation and characteriz ...
Panax quinquefolius
Funct. Integr. Genomics
14
545-557
2014
-
-
1
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
1
-
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1
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2
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1
2
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1
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1
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1
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-
725871
Dai
Metabolic engineering of Sacch ...
Panax ginseng
Metab. Eng.
20
146-156
2013
-
-
1
-
-
-
-
-
-
-
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1
-
5
-
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1
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1
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1
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1
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-
726170
Sun
Regulation and differential ex ...
Panax ginseng, Panax quinquefolius
Plant Growth Regul.
71
207-217
2013
-
-
1
-
-
-
-
-
-
-
1
2
-
2
-
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1
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2
1
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1
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1
2
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1
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2
1
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726145
Han
Cytochrome P450 CYP716A53v2 ca ...
Panax ginseng
Plant Cell Physiol.
53
1535-1545
2012
-
-
-
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1
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4
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3
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1
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1
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3
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1
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1
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1
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726142
Han
The Cyt P450 enzyme CYP716A47 ...
Panax ginseng
Plant Cell Physiol.
52
2062-2073
2011
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1
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1
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4
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1
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1
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