BRENDA - Enzyme Database show
show all sequences of 1.14.14.121

Cytochrome P450 CYP716A53v2 catalyzes the formation of protopanaxatriol from protopanaxadiol during ginsenoside biosynthesis in Panax ginseng

Han, J.Y.; Hwang, H.S.; Choi, S.W.; Kim, H.J.; Choi, Y.E.; Plant Cell Physiol. 53, 1535-1545 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Saccharomyces cerevisiae WAT21 cells
Panax ginseng
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55300
-
x * 55300, calculated from amino acid sequence
Panax ginseng
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
protopanaxadiol + [reduced NADPH-hemoprotein reductase] + O2
Panax ginseng
-
protopanaxatriol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Panax ginseng
I7C6E8
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower bud
-
Panax ginseng
-
leaf
-
Panax ginseng
-
petiole
-
Panax ginseng
-
rhizome
highest expression
Panax ginseng
-
root
-
Panax ginseng
-
sympodium
-
Panax ginseng
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
protopanaxadiol + [reduced NADPH-hemoprotein reductase] + O2
-
726145
Panax ginseng
protopanaxatriol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
ir
Subunits
Subunits
Commentary
Organism
?
x * 55300, calculated from amino acid sequence
Panax ginseng
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Saccharomyces cerevisiae WAT21 cells
Panax ginseng
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55300
-
x * 55300, calculated from amino acid sequence
Panax ginseng
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
protopanaxadiol + [reduced NADPH-hemoprotein reductase] + O2
Panax ginseng
-
protopanaxatriol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
ir
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower bud
-
Panax ginseng
-
leaf
-
Panax ginseng
-
petiole
-
Panax ginseng
-
rhizome
highest expression
Panax ginseng
-
root
-
Panax ginseng
-
sympodium
-
Panax ginseng
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
protopanaxadiol + [reduced NADPH-hemoprotein reductase] + O2
-
726145
Panax ginseng
protopanaxatriol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
ir
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 55300, calculated from amino acid sequence
Panax ginseng
Expression
Organism
Commentary
Expression
Panax ginseng
the enzyme is not affected by methyl jasmonate treatment
additional information
General Information
General Information
Commentary
Organism
metabolism
protopanaxadiol 6-hydroxylase produces protopanaxatriol from protopanaxadiol, which is an important step in the formation of dammarane-type triterpene aglycones in ginseng saponin biosynthesis
Panax ginseng
General Information (protein specific)
General Information
Commentary
Organism
metabolism
protopanaxadiol 6-hydroxylase produces protopanaxatriol from protopanaxadiol, which is an important step in the formation of dammarane-type triterpene aglycones in ginseng saponin biosynthesis
Panax ginseng
Expression (protein specific)
Organism
Commentary
Expression
Panax ginseng
the enzyme is not affected by methyl jasmonate treatment
additional information
Other publictions for EC 1.14.14.121
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745480
Park
Alteration of Panax ginseng s ...
Panax ginseng
J. Ginseng Res.
40
47-54
2016
-
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
726485
Dai
Producing aglycons of ginsenos ...
Panax ginseng
Sci. Rep.
4
3698
2014
-
-
1
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
745012
Wang
Identification of the protopa ...
Panax quinquefolius
Funct. Integr. Genomics
14
559-570
2014
-
-
1
-
1
-
-
-
-
-
-
1
-
5
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-
726145
Han
Cytochrome P450 CYP716A53v2 ca ...
Panax ginseng
Plant Cell Physiol.
53
1535-1545
2012
-
-
1
-
-
-
-
-
-
-
1
1
-
4
-
-
-
-
-
6
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
6
-
-
1
1
-
-
-
-
-
-
-
-
1
1
1
1
-
-
724615
Yue
Protopanaxadiol 6-hydroxylase ...
Panax notoginseng
Biotechnol. Bioeng.
100
933-940
2008
1
-
-
-
-
-
5
1
-
-
-
2
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
5
-
1
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-