BRENDA - Enzyme Database show
show all sequences of 1.14.14.137

High humidity induces abscisic acid 8-hydroxylase in stomata and vasculature to regulate local and systemic abscisic acid responses in Arabidopsis

Okamoto, M.; Tanaka, Y.; Abrams, S.R.; Kamiya, Y.; Seki, M.; Nambara, E.; Plant Physiol. 149, 825-834 (2009)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
high humidity reduces the abscisic acid levels by the activation of CYP707A1 and CYP707A3
Arabidopsis thaliana
Cloned(Commentary)
Commentary
Organism
promoter fragment of the translational start of each CYP707A amplified and cloned into pENTR/D-TOPO vector, cloned into the binary vector, pGWB3, with a recombination cassette for the expression of GUS-fused protein. Resulting plasmids electroporated into Agrobacterium strain GV3101, which is used to transform wild-type Arabidopsis accession Columbia plants
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
guard cell
major site of CYP707A1 and CYP707A3 expression
Arabidopsis thaliana
-
shoot
CYP707A3 plays a major role in regulating abscisic acid levels, whereas CYP707A1 plays a minor role in regulating abscisic acid levels in shoots
Arabidopsis thaliana
-
stoma
-
Arabidopsis thaliana
-
vascular tissue
major site of CYP707A1 and CYP707A3 expression
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-abscisate + [reduced NADPH-hemoprotein reductase] + O2
-
706300
Arabidopsis thaliana
8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
high humidity reduces the abscisic acid levels by the activation of CYP707A1 and CYP707A3
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
promoter fragment of the translational start of each CYP707A amplified and cloned into pENTR/D-TOPO vector, cloned into the binary vector, pGWB3, with a recombination cassette for the expression of GUS-fused protein. Resulting plasmids electroporated into Agrobacterium strain GV3101, which is used to transform wild-type Arabidopsis accession Columbia plants
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
guard cell
major site of CYP707A1 and CYP707A3 expression
Arabidopsis thaliana
-
shoot
CYP707A3 plays a major role in regulating abscisic acid levels, whereas CYP707A1 plays a minor role in regulating abscisic acid levels in shoots
Arabidopsis thaliana
-
stoma
-
Arabidopsis thaliana
-
vascular tissue
major site of CYP707A1 and CYP707A3 expression
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-abscisate + [reduced NADPH-hemoprotein reductase] + O2
-
706300
Arabidopsis thaliana
8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Expression
Organism
Commentary
Expression
Arabidopsis thaliana
when wild-type plants are transferred to high-humidity conditions (relative humidity 90%), CYP707A1 and CYP707A3 transcript levels increase primarily in guard cells and vascular tissues, respectively
up
General Information
General Information
Commentary
Organism
physiological function
CYP707A1 and CYP707A3 are involved in stomatal opening in response to high humidity. Cyp707a1 and cyp707a3 mutants display lower stomatal conductance under turgid conditions (relative humidity 60%) than the wild-type. Cyp707a3 mutant exhibits high abscisic acid levels even after transferring to high-humidity conditions, whereas, under similar conditions, the cyp707a1 mutant exhibits low abscisic acid levels comparable to the wild-type. Stomatal closure of the cyp707a1 mutant, but not cyp707a3 mutant, is abscisic acid hypersensitive when epidermal peel ias treated with exogenous abscisic acid. CYP707A3 reduces the amount of mobile abscisic acid in vascular tissues in response to high humidity, whereas CYP707A1 inactivates local abscisic acid pools inside the guard cells
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
physiological function
CYP707A1 and CYP707A3 are involved in stomatal opening in response to high humidity. Cyp707a1 and cyp707a3 mutants display lower stomatal conductance under turgid conditions (relative humidity 60%) than the wild-type. Cyp707a3 mutant exhibits high abscisic acid levels even after transferring to high-humidity conditions, whereas, under similar conditions, the cyp707a1 mutant exhibits low abscisic acid levels comparable to the wild-type. Stomatal closure of the cyp707a1 mutant, but not cyp707a3 mutant, is abscisic acid hypersensitive when epidermal peel ias treated with exogenous abscisic acid. CYP707A3 reduces the amount of mobile abscisic acid in vascular tissues in response to high humidity, whereas CYP707A1 inactivates local abscisic acid pools inside the guard cells
Arabidopsis thaliana
Expression (protein specific)
Organism
Commentary
Expression
Arabidopsis thaliana
when wild-type plants are transferred to high-humidity conditions (relative humidity 90%), CYP707A1 and CYP707A3 transcript levels increase primarily in guard cells and vascular tissues, respectively
up
Other publictions for EC 1.14.14.137
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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6
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8
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14
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39
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3
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5
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3
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706300
Okamoto
High humidity induces abscisic ...
Arabidopsis thaliana
Plant Physiol.
149
825-834
2009
1
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706301
Barrero
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Hordeum vulgare
Plant Physiol.
150
1006-1021
2009
1
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1
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1
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Nicotiana plumbaginifolia
Plant Physiol.
150
1556-1566
2009
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1
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706360
Liu
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Arabidopsis thaliana
Plant Signal. Behav.
4
905-907
2009
1
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1
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706376
Nitsch
Abscisic acid levels in tomato ...
Solanum lycopersicum
Planta
229
1335-1346
2009
3
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1
1
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1
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685537
Todoroki
Structure-activity relationshi ...
Arabidopsis thaliana
Bioorg. Med. Chem.
16
3141-3152
2008
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10
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1
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6
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10
6
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1
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1
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