BRENDA - Enzyme Database show
show all sequences of 1.14.14.142

8-dimethylallylnaringenin 2'-hydroxylase, the crucial cytochrome P450 mono-oxygenase for lavandulylated flavanone formation in Sophora flavescens cultured cells

Yamamoto, H.; Yatou, A.; Inoue, K.; Phytochemistry 58, 671-676 (2001)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
carbon monoxide
-
Sophora flavescens
cytochrome c
70% inhibition at 10 mM
Sophora flavescens
Metyrapone
34% inhibition at 1 mM
Sophora flavescens
miconazole
36% inhibition at 1 mM
Sophora flavescens
additional information
no or poor inhibition by ancymidol and KCN
Sophora flavescens
tropolon
30% inhibition at 1 mM
Sophora flavescens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.055
-
8-dimethylallylnaringenin
-
Sophora flavescens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
the enzyme is tightly bound to the membrane
Sophora flavescens
16020
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
8-dimethylallylnaringenin + [reduced NADPH-hemoprotein reductase] + O2
Sophora flavescens
the enzyme is crucial for lavandulylated flavanone formation, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN, which is then formed to sophoraflavanone G, pathway overview
leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sophora flavescens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Sophora flavescens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(-)-(2S)-8-dimethylallylnaringenin + [oxidized NADPH-hemoprotein reductase] + H2O
i.e. sophoraflavanone B, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN and is O2-dependent
676359
Sophora flavescens
leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
8-dimethylallylnaringenin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme is crucial for lavandulylated flavanone formation, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN, which is then formed to sophoraflavanone G, pathway overview
676359
Sophora flavescens
leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
the enzyme is a cytochrome P450 monooxygenase
676359
Sophora flavescens
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Sophora flavescens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Sophora flavescens
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
the enzyme is a cytochrome P450 monooxygenase
Sophora flavescens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
the enzyme is a cytochrome P450 monooxygenase
Sophora flavescens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
carbon monoxide
-
Sophora flavescens
cytochrome c
70% inhibition at 10 mM
Sophora flavescens
Metyrapone
34% inhibition at 1 mM
Sophora flavescens
miconazole
36% inhibition at 1 mM
Sophora flavescens
additional information
no or poor inhibition by ancymidol and KCN
Sophora flavescens
tropolon
30% inhibition at 1 mM
Sophora flavescens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.055
-
8-dimethylallylnaringenin
-
Sophora flavescens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
the enzyme is tightly bound to the membrane
Sophora flavescens
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
8-dimethylallylnaringenin + [reduced NADPH-hemoprotein reductase] + O2
Sophora flavescens
the enzyme is crucial for lavandulylated flavanone formation, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN, which is then formed to sophoraflavanone G, pathway overview
leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Sophora flavescens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(-)-(2S)-8-dimethylallylnaringenin + [oxidized NADPH-hemoprotein reductase] + H2O
i.e. sophoraflavanone B, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN and is O2-dependent
676359
Sophora flavescens
leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
8-dimethylallylnaringenin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme is crucial for lavandulylated flavanone formation, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN, which is then formed to sophoraflavanone G, pathway overview
676359
Sophora flavescens
leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
the enzyme is a cytochrome P450 monooxygenase
676359
Sophora flavescens
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Sophora flavescens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Sophora flavescens
Other publictions for EC 1.14.14.142
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
676566
Zhao
Characterization of leachianon ...
Sophora flavescens
Plant Physiol.
133
1306-1313
2003
-
-
-
-
-
-
-
-
2
-
-
1
-
3
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676359
Yamamoto
8-dimethylallylnaringenin 2'-h ...
Sophora flavescens
Phytochemistry
58
671-676
2001
-
-
-
-
-
-
6
1
1
-
-
1
-
2
-
-
-
-
-
1
-
-
3
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
6
-
1
1
-
-
1
-
-
-
-
-
1
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-