BRENDA - Enzyme Database show
show all sequences of 1.14.14.145

Diterpenoid resin acid biosynthesis in conifers: characterization of two cytochrome P450-dependent monooxygenases and an aldehyde dehydrogenase involved in abietic acid biosynthesis

Funk, C.; Croteau, R.; Arch. Biochem. Biophys. 308, 258-266 (1994)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ancymidol
-
Abies grandis
clotrimazole
-
Abies grandis
CO
blue light reversible inhibition
Abies grandis
cytochrome c
-
Abies grandis
Metyrapone
-
Abies grandis
miconazole
-
Abies grandis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
abietadienol
pH 7.5, 32°C
Abies grandis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Abies grandis
-
-
microsome
-
Pinus contorta
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
abieta-7,13-dien-18-ol + [reduced NADPH-hemoprotein reductase] + O2
Pinus contorta
this enzyme catalyzes a step in the pathway of abietic acid biosynthesis
abieta-7,13-dien-18-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
abieta-7,13-dien-18-ol + [reduced NADPH-hemoprotein reductase] + O2
Abies grandis
this enzyme catalyzes a step in the pathway of abietic acid biosynthesis. Hydroxylation activity is maximally supported by NADPH, and NADH is less effective as reductant
abieta-7,13-dien-18-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Abies grandis
-
Lindl.
-
Pinus contorta
-
Douglas var. latifolia Engelman
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
stem
cell-free stem extracts
Abies grandis
-
stem
cell-free stem extracts
Pinus contorta
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
abieta-7,13-dien-18-ol + [reduced NADPH-hemoprotein reductase] + O2
this enzyme catalyzes a step in the pathway of abietic acid biosynthesis
690605
Pinus contorta
abieta-7,13-dien-18-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
abieta-7,13-dien-18-ol + [reduced NADPH-hemoprotein reductase] + O2
this enzyme catalyzes a step in the pathway of abietic acid biosynthesis. Hydroxylation activity is maximally supported by NADPH, and NADH is less effective as reductant
690605
Abies grandis
abieta-7,13-dien-18-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
32
-
assay at
Abies grandis
32
-
assay at
Pinus contorta
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
7.6
-
Abies grandis
7.6
-
assay at
Pinus contorta
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
8
60-70% of maximal activity at pH 7.0 and 8.0
Abies grandis
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
cytochrome P-450-dependent monooxygenase
Abies grandis
FAD
omission of flavins results in measurable reduction in both hydroxylation activities
Abies grandis
FMN
omission of flavins results in measurable reduction in both hydroxylation activities
Abies grandis
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
additional information
-
no inhibition detected by ancymidol at 0.2 mM
Abies grandis
additional information
0.0013
-
pH 7.5, 32°C
Abies grandis
clotrimazole
0.004
-
pH 7.5, 32°C
Abies grandis
cytochrome c
0.005
-
pH 7.5, 32°C
Abies grandis
Metyrapone
0.307
-
pH 7.5, 32°C
Abies grandis
miconazole
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
cytochrome P-450-dependent monooxygenase
Abies grandis
FAD
omission of flavins results in measurable reduction in both hydroxylation activities
Abies grandis
FMN
omission of flavins results in measurable reduction in both hydroxylation activities
Abies grandis
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
additional information
-
no inhibition detected by ancymidol at 0.2 mM
Abies grandis
additional information
0.0013
-
pH 7.5, 32°C
Abies grandis
clotrimazole
0.004
-
pH 7.5, 32°C
Abies grandis
cytochrome c
0.005
-
pH 7.5, 32°C
Abies grandis
Metyrapone
0.307
-
pH 7.5, 32°C
Abies grandis
miconazole
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ancymidol
-
Abies grandis
clotrimazole
-
Abies grandis
CO
blue light reversible inhibition
Abies grandis
cytochrome c
-
Abies grandis
Metyrapone
-
Abies grandis
miconazole
-
Abies grandis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
abietadienol
pH 7.5, 32°C
Abies grandis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Abies grandis
-
-
microsome
-
Pinus contorta
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
abieta-7,13-dien-18-ol + [reduced NADPH-hemoprotein reductase] + O2
Pinus contorta
this enzyme catalyzes a step in the pathway of abietic acid biosynthesis
abieta-7,13-dien-18-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
abieta-7,13-dien-18-ol + [reduced NADPH-hemoprotein reductase] + O2
Abies grandis
this enzyme catalyzes a step in the pathway of abietic acid biosynthesis. Hydroxylation activity is maximally supported by NADPH, and NADH is less effective as reductant
abieta-7,13-dien-18-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
stem
cell-free stem extracts
Abies grandis
-
stem
cell-free stem extracts
Pinus contorta
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
abieta-7,13-dien-18-ol + [reduced NADPH-hemoprotein reductase] + O2
this enzyme catalyzes a step in the pathway of abietic acid biosynthesis
690605
Pinus contorta
abieta-7,13-dien-18-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
abieta-7,13-dien-18-ol + [reduced NADPH-hemoprotein reductase] + O2
this enzyme catalyzes a step in the pathway of abietic acid biosynthesis. Hydroxylation activity is maximally supported by NADPH, and NADH is less effective as reductant
690605
Abies grandis
abieta-7,13-dien-18-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
32
-
assay at
Abies grandis
32
-
assay at
Pinus contorta
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
7.6
-
Abies grandis
7.6
-
assay at
Pinus contorta
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
8
60-70% of maximal activity at pH 7.0 and 8.0
Abies grandis
Other publictions for EC 1.14.14.145
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
666567
Ro
Diterpene resin acid biosynthe ...
Pinus taeda
Phytochemistry
67
1572-1578
2006
-
-
1
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676795
Ro
Loblolly pine abietadienol/abi ...
Picea sitchensis, Pinus taeda
Proc. Natl. Acad. Sci. USA
102
8060-8065
2005
1
-
2
-
-
-
-
9
2
-
-
9
-
11
-
-
-
-
-
2
1
-
25
-
1
-
-
-
2
1
-
1
-
-
-
1
-
7
1
-
-
-
-
-
-
15
7
-
-
9
-
-
-
-
-
2
1
-
25
-
2
-
-
-
2
1
-
-
-
-
-
-
-
-
690605
Funk
Diterpenoid resin acid biosynt ...
Abies grandis, Pinus contorta
Arch. Biochem. Biophys.
308
258-266
1994
-
-
-
-
-
-
6
1
2
-
-
2
-
2
-
-
-
-
-
2
-
-
2
-
2
-
-
-
2
1
-
3
-
-
5
-
-
-
3
-
-
-
5
6
-
1
2
-
-
2
-
-
-
-
-
2
-
-
2
-
2
-
-
-
2
1
-
-
-
-
-
-
-
-
694704
Funk
Regulation of oleoresinosis in ...
Abies grandis
Plant Physiol.
106
999-1005
1994
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-