Application | Comment | Organism |
---|---|---|
agriculture | target enzyme for azole antifungal agents. These specific inhibitors are of great importance as plant growth regulators, fungicides and herbicides in the agricultural and medical fields | Zea mays |
agriculture | target of important agrochemicals such as fungicides, plant growth regulators and herbicides | Zea mays |
medicine | target enzyme for azole antifungal agents. These specific inhibitors are of great importance as plant growth regulators, fungicides and herbicides in the agricultural and medical fields | Zea mays |
pharmacology | target enzyme for azole antifungal agents. These specific inhibitors are of great importance as plant growth regulators, fungicides and herbicides in the agricultural and medical fields | Zea mays |
General Stability | Organism |
---|---|
plant demethylase is remarkably stable | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters | Zea mays | |
0.116 | - |
(3beta,4alpha,5alpha)-4,14-dimethylcholest-8-en-3-ol | - |
Zea mays | |
0.16 | - |
obtusifoliol | - |
Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Zea mays | 5783 | - |
endoplasmic reticulum | light membrane fraction of endoplasmic reticulum, not in plasma membrane | Zea mays | 5783 | - |
membrane | membrane-bound | Zea mays | 16020 | - |
microsome | microsomal-bound | Zea mays | - |
- |
additional information | not in plasma membrane | Zea mays | - |
- |
additional information | subcellular localization, distribution | Zea mays | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Zea mays | enzyme of sterol biosynthetic pathway | ? | - |
? | |
additional information | Zea mays | enzyme of plant sterol, phytosterol, biosynthesis | ? | - |
? | |
obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | Zea mays | 4alpha,14alpha-dimethyl-24-methylene-5alpha-cholesta-8-en-3beta-ol | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | Zea mays | 4alpha,14alpha-dimethyl-5alpha-ergosta-8,24(28)-dien-3beta-ol | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
maize | - |
Zea mays | - |
LG11 | - |
Zea mays LG11 | - |
LG11 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 14alpha-methylsteroid + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 = a DELTA14-steroid + formate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | mechanism | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3b,4a,5a)-4,14-dimethylcholest-8-en-3-ol + [reduced NADPH-hemoprotein reductase] + O2 | good substrate, 67% of activity compared to obtusifoliol | Zea mays | ? + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
(3beta,4alpha,5alpha)-4,14-dimethylcholest-8-en-3-ol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Zea mays | ? | - |
r | |
14alpha-methyl-24,28-dihydrofecosterol + [reduced NADPH-hemoprotein reductase] + O2 | 50% of activity compared to obtusifoliol | Zea mays | ? + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
24,28-dihydroobtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | good substrate, 75% of activity to obtusifoliol | Zea mays | 4alpha-methyl-5alpha-ergosta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
24,28-dihydroobtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | DHO | Zea mays | 4alpha-methyl-5alpha-ergosta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
24,28-dihydroobtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | 4alpha,14alpha-dimethyl-5alpha-ergosta-8-en-3beta-ol | Zea mays | 4alpha-methyl-5alpha-ergosta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | substrate specificity | Zea mays | ? | - |
? | |
additional information | no activity with 31-norlanosterol, cycloeucalenol, 4alpha,14alpha-dimethyl-5alpha-ergost-9(11)-en-3beta-ol, 4alpha,14alpha-dimethyl-5alpha-ergost-7-en-3beta-ol, 8(9),24(25)-tetrahydro-31-norlanosterol, 24-methylenelanosterol, 24,28-dihydro-4beta-methyl-30-norobtusifoliol, 24,25-dihydrolanosterol, lanosterol, obtusifoliyl-3beta-methoxy, obtusifoliyl-3beta-acetoxy, obtusifoliyl-3beta-amino | Zea mays | ? | - |
? | |
additional information | biosynthetic enzyme with very narrow substrate specificity | Zea mays | ? | - |
? | |
additional information | P-450OBT 14DM has probably a specific apolar binding site for the side chain. DELTA8-double bond is absolute required for substrate demethylation and the 3-hydroxy group plays a critical role in enzyme-substrate interaction | Zea mays | ? | - |
? | |
additional information | enzyme with high degree of substrate and product specificity | Zea mays | ? | - |
? | |
additional information | plant sterol 14alpha-demethylase have high substrate specificity | Zea mays | ? | - |
? | |
additional information | narrow substrate selectivity | Zea mays | ? | - |
? | |
additional information | enzyme of sterol biosynthetic pathway | Zea mays | ? | - |
? | |
additional information | enzyme of plant sterol, phytosterol, biosynthesis | Zea mays | ? | - |
? | |
additional information | substrate specificity | Zea mays LG11 | ? | - |
? | |
additional information | no activity with 31-norlanosterol, cycloeucalenol, 4alpha,14alpha-dimethyl-5alpha-ergost-9(11)-en-3beta-ol, 4alpha,14alpha-dimethyl-5alpha-ergost-7-en-3beta-ol, 8(9),24(25)-tetrahydro-31-norlanosterol, 24-methylenelanosterol, 24,28-dihydro-4beta-methyl-30-norobtusifoliol, 24,25-dihydrolanosterol, lanosterol, obtusifoliyl-3beta-methoxy, obtusifoliyl-3beta-acetoxy, obtusifoliyl-3beta-amino | Zea mays LG11 | ? | - |
? | |
additional information | biosynthetic enzyme with very narrow substrate specificity | Zea mays LG11 | ? | - |
? | |
additional information | P-450OBT 14DM has probably a specific apolar binding site for the side chain. DELTA8-double bond is absolute required for substrate demethylation and the 3-hydroxy group plays a critical role in enzyme-substrate interaction | Zea mays LG11 | ? | - |
? | |
additional information | enzyme with high degree of substrate and product specificity | Zea mays LG11 | ? | - |
? | |
obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | best substrate | Zea mays | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | 4alpha,14alpha-dimethyl-24-methylene-5alpha-cholesta-8-en-3beta-ol | Zea mays | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | catalyzes 14alpha-demethylation of obtusifoliol | Zea mays | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | 4alpha,14alpha-dimethyl-5alpha-ergosta-8,24(28)-dien-3beta-ol | Zea mays | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Zea mays |
30 | - |
aerobic conditions | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Zea mays |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | - |
Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Zea mays | |
heme | heme-thiolate enzyme | Zea mays |