BRENDA - Enzyme Database show
show all sequences of 1.14.14.156

Camalexin is synthesized from indole-3-acetaldoxime, a key branching point between primary and secondary metabolism in Arabidopsis

Glawischnig, E.; Hansen, B.; Olsen, C.; Halkier, B.; Proc. Natl. Acad. Sci. USA 101, 8245-8250 (2004)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-tryptophan + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
716733
Arabidopsis thaliana
(E)-indol-3-ylacetaldoxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-tryptophan + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
716733
Arabidopsis thaliana
(E)-indol-3-ylacetaldoxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O
-
-
-
?
Expression
Organism
Commentary
Expression
Arabidopsis thaliana
the transcript level of CYP79B2, but not CYP79B3, is increased 3fold upon induction of camalexin by silver nitrate
up
General Information
General Information
Commentary
Organism
malfunction
the cyp79B2/cyp79B3 double knockout mutant is devoid of camalexin, as it is also devoid of indole glucosinolates
Arabidopsis thaliana
metabolism
CYP79B2 plays an important role in camalexin biosynthesis
Arabidopsis thaliana
physiological function
only CYP79B2 and CYP79B3 contribute significantly to the indole-3-acetaldoxime pool from which camalexin and indole glucosinolates are synthesized
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
malfunction
the cyp79B2/cyp79B3 double knockout mutant is devoid of camalexin, as it is also devoid of indole glucosinolates
Arabidopsis thaliana
metabolism
CYP79B2 plays an important role in camalexin biosynthesis
Arabidopsis thaliana
physiological function
only CYP79B2 and CYP79B3 contribute significantly to the indole-3-acetaldoxime pool from which camalexin and indole glucosinolates are synthesized
Arabidopsis thaliana
Expression (protein specific)
Organism
Commentary
Expression
Arabidopsis thaliana
the transcript level of CYP79B2, but not CYP79B3, is increased 3fold upon induction of camalexin by silver nitrate
up
Other publictions for EC 1.14.14.156
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
716536
Sanchez-Vallet
Tryptophan-derived secondary m ...
Arabidopsis thaliana
Plant J.
63
115-127
2010
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6
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1
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1
1
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714117
Glawischnig
The role of cytochrome P450 en ...
Arabidopsis thaliana
Biochem. Soc. Trans.
34
1206-1208
2006
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4
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1
1
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716733
Glawischnig
Camalexin is synthesized from ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
101
8245-8250
2004
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1
3
3
1
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713941
Naur
CYP79B1 from Sinapis alba conv ...
Sinapis alba
Arch. Biochem. Biophys.
409
235-241
2003
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1
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1
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5
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716592
Mikkelsen
Modulation of CYP79 genes and ...
Arabidopsis thaliana
Plant Physiol.
131
298-308
2003
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3
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2
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2
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715111
Zhao
Trp-dependent auxin biosynthes ...
Arabidopsis thaliana
Genes Dev.
16
3100-3112
2002
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3
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2
2
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715448
Mikkelsen
Cytochrome P450 CYP79B2 from A ...
Arabidopsis thaliana
J. Biol. Chem.
275
33712-33717
2000
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1
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6
1
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2
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4
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4
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1
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6
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1
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1
1
1
1
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716797
Hull
Arabidopsis cytochrome P450s t ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
97
2379-2384
2000
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1
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2
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