BRENDA - Enzyme Database show
show all sequences of 1.14.14.24

Metabolism of vitamin D by human microsomal CYP2R1

Shinkyo, R.; Sakaki, T.; Kamakura, M.; Ohta, M.; Inouye, K.; Biochem. Biophys. Res. Commun. 324, 451-457 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Saccharomyces cerevisiae
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00045
-
vitamin D2
pH 7.4, 37°C
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Homo sapiens
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
addition of NAD+ in presence of 1alpha-hydroxy-vitamin D2 to CYP2R1 induces a typical type I spectrum, indicating a change of the heme iron of CYP2R1 from a low-spin state to a high-spin state
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1alpha-hydroxy-vitamin D2 + O2 + [reduced NADPH-hemoprotein reductase] + H+
-
717179
Homo sapiens
1alpha,25-dihydroxy-vitamin D2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1alpha-hydroxy-vitamin D3 + O2 + [reduced NADPH-hemoprotein reductase] + H+
-
717179
Homo sapiens
1alpha,25-dihydroxy-vitamin D3 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
CYP2R1 shows no detectable 1alpha-hydroxylation activity
717179
Homo sapiens
?
-
-
-
-
vitamin D2 + O2 + NADPH + H+
-
717179
Homo sapiens
25-hydroxy-vitamin D2 + NADP+ + H2O
-
-
-
?
vitamin D3 + O2 + NADPH + H+
-
717179
Homo sapiens
25-hydroxy-vitamin D3 + NADP+ + H2O
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.017
-
vitamin D2
pH 7.4, 37°C
Homo sapiens
Cofactor
Cofactor
Commentary
Organism
Structure
heme
addition of NAD+ in presence of 1alpha-hydroxy-vitamin D2 to CYP2R1 induces a typical type I spectrum, indicating a change of the heme iron of CYP2R1 from a low-spin state to a high-spin state
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cerevisiae
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme
addition of NAD+ in presence of 1alpha-hydroxy-vitamin D2 to CYP2R1 induces a typical type I spectrum, indicating a change of the heme iron of CYP2R1 from a low-spin state to a high-spin state
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00045
-
vitamin D2
pH 7.4, 37°C
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Homo sapiens
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
addition of NAD+ in presence of 1alpha-hydroxy-vitamin D2 to CYP2R1 induces a typical type I spectrum, indicating a change of the heme iron of CYP2R1 from a low-spin state to a high-spin state
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1alpha-hydroxy-vitamin D2 + O2 + [reduced NADPH-hemoprotein reductase] + H+
-
717179
Homo sapiens
1alpha,25-dihydroxy-vitamin D2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1alpha-hydroxy-vitamin D3 + O2 + [reduced NADPH-hemoprotein reductase] + H+
-
717179
Homo sapiens
1alpha,25-dihydroxy-vitamin D3 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
CYP2R1 shows no detectable 1alpha-hydroxylation activity
717179
Homo sapiens
?
-
-
-
-
vitamin D2 + O2 + NADPH + H+
-
717179
Homo sapiens
25-hydroxy-vitamin D2 + NADP+ + H2O
-
-
-
?
vitamin D3 + O2 + NADPH + H+
-
717179
Homo sapiens
25-hydroxy-vitamin D3 + NADP+ + H2O
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.017
-
vitamin D2
pH 7.4, 37°C
Homo sapiens
Other publictions for EC 1.14.14.24
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735419
Nissen
Common variants in CYP2R1 and ...
Homo sapiens
Am. J. Clin. Nutr.
101
218-227
2015
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1
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1
1
-
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-
736019
De Toni
Uncarboxylated osteocalcin sti ...
Mus musculus
Endocrinology
155
4266-4274
2014
-
-
-
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-
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3
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2
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1
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1
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-
1
1
1
1
-
-
736222
Sundaram
1alpha,25-dihydroxyvitamin D3 ...
Homo sapiens
Horm. Cancer
5
90-97
2014
-
1
1
-
-
-
-
-
1
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1
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2
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4
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1
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2
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1
1
2
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1
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1
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4
-
-
1
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-
-
-
-
-
-
-
1
1
1
1
-
-
724907
Urbschat
Vitamin D hydroxylases CYP2R1, ...
Homo sapiens
Eur. J. Clin. Invest.
43
1282-1290
2013
-
1
-
-
-
-
-
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-
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1
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1
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-
-
-
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-
-
1
-
-
1
-
-
735593
Yasuda
UV-dependent production of 25- ...
Homo sapiens
Biochem. Biophys. Res. Commun.
434
311-315
2013
-
-
1
-
-
-
-
-
1
-
-
2
-
3
-
-
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-
-
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-
3
-
1
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1
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2
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1
2
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1
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-
2
-
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-
-
-
3
-
1
-
-
-
1
-
-
-
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1
1
-
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-
737160
Zhu
CYP2R1 is a major, but not exc ...
Mus musculus, Mus musculus C57BL/6
Proc. Natl. Acad. Sci. USA
110
15650-15655
2013
-
-
1
-
1
-
-
-
-
-
-
2
-
3
-
-
-
-
-
3
-
-
2
-
1
-
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-
2
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-
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-
1
2
-
1
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-
-
-
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-
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-
2
-
-
-
-
-
3
-
-
2
-
1
-
-
-
-
-
-
-
-
2
2
-
-
-
718332
Wang
Development of experimental au ...
Mus musculus
Proc. Natl. Acad. Sci. USA
109
8501-8504
2012
-
1
-
-
-
-
-
-
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-
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2
-
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1
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-
1
1
-
-
-
717838
Kaufmann
Bioengineering anabolic vitami ...
Homo sapiens
J. Biol. Chem.
286
28729-28737
2011
-
-
-
1
3
-
-
-
-
-
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3
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1
3
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-
-
-
-
725609
Foresta
Bone mineral density and testi ...
Homo sapiens
J. Clin. Endocrinol. Metab.
96
E646-E652
2011
-
1
-
-
-
-
-
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2
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3
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1
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3
-
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-
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-
717180
Fujii
Purification, characterization ...
Pseudonocardia autotrophica, Pseudonocardia autotrophica NBRC 12743
Biochem. Biophys. Res. Commun.
385
170-175
2009
3
-
1
-
6
-
-
3
-
-
1
-
-
2
-
-
-
-
-
-
-
-
4
1
1
-
-
-
1
-
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-
-
-
-
3
-
1
-
-
6
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
717988
Strushkevich
Structural analysis of CYP2R1 ...
Homo sapiens
J. Mol. Biol.
380
95-106
2008
-
-
1
1
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
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3
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1
-
1
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3
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4
-
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3
-
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718401
Aiba
Characterization of rat and hu ...
Homo sapiens, Rattus norvegicus
Steroids
71
849-856
2006
-
-
2
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-
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3
-
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5
-
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8
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6
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2
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2
2
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3
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8
-
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-
-
6
-
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-
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-
-
-
-
717071
Rahmaniyan
Characterization of recombinan ...
Rattus norvegicus
Am. J. Physiol. Endocrinol. Metab.
288
E753-E760
2005
-
-
1
-
-
-
-
5
1
-
-
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2
-
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1
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7
-
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1
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5
1
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-
-
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-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
717179
Shinkyo
Metabolism of vitamin D by hum ...
Homo sapiens
Biochem. Biophys. Res. Commun.
324
451-457
2004
-
-
1
-
-
-
-
1
1
1
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2
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5
-
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1
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1
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1
1
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1
1
1
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-
5
-
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-
-
1
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717769
Yamasaki
Identification of a novel rat ...
Rattus norvegicus
J. Biol. Chem.
279
22848-22856
2004
-
-
1
-
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-
2
2
1
-
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-
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3
-
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3
1
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3
1
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-
-
2
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1
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1
1
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2
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2
1
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3
1
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3
1
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2
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1
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1
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718313
Cheng
Genetic evidence that the huma ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
101
7711-7715
2004
-
1
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1
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1
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2
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1
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717767
Cheng
De-orphanization of cytochrome ...
Mus musculus
J. Biol. Chem.
278
38084-38093
2003
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1
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1
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2
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2
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1
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1
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2
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1
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1
1
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717177
Hosseinpour
The importance of residues in ...
Sus scrofa
Biochem. Biophys. Res. Commun.
288
1059-1063
2001
-
-
1
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1
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2
1
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4
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4
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1
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1
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1
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4
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717176
Postlind
Cloning, structure, and expres ...
Sus scrofa
Biochem. Biophys. Res. Commun.
241
491-497
1997
-
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1
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-
-
-
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2
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1
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2
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1
1
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1
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2
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2
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1
1
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-
-
-
-
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-
-
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717599
Axen
Purification from pig kidney o ...
Sus scrofa
FEBS Lett.
375
277-279
1995
-
-
-
-
-
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-
1
-
1
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1
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1
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1
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2
1
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1
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1
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1
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1
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1
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1
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2
1
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717750
Masumoto
Purification and characterizat ...
Rattus norvegicus
J. Biol. Chem.
263
14256-14260
1988
-
-
-
-
-
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2
1
2
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1
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2
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1
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2
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3
1
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1
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3
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3
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2
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1
2
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1
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1
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2
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3
1
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1
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717287
Saarem
25-Hydroxylation of 1 alpha-hy ...
Rattus norvegicus
Biochim. Biophys. Acta
840
117-126
1985
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