BRENDA - Enzyme Database show
show all sequences of 1.14.14.43

The involvement of two p450 enzymes, CYP83B1 and CYP83A1, in auxin homeostasis and glucosinolate biosynthesis

Bak, S.; Feyereisen, R.; Plant Physiol. 127, 108-118 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Saccharomyces cerevisiae
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
indole-3-acetaldoxime
pH not specified in the publication, temperature not specified in the publication
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
P48421
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(E)-4-hydroxyphenylacetaldoxime + O2 + [reduced NADPH-hemoprotein reductase]
-
741195
Arabidopsis thaliana
1-aci-nitro-2-(4-hydroxyphenyl)-ethane + H2O + [oxidized NADPH-hemoprotein reductase]
first step of reaction
-
-
?
(E)-phenylacetaldoxime + O2 + [reduced NADPH-hemoprotein reductase]
-
741195
Arabidopsis thaliana
1-aci-nitro-2-phenylethane + H2O + [oxidized NADPH-hemoprotein reductase]
first step of reaction
-
-
?
1-aci-nitro-2-(4-hydroxyphenyl)-ethane + 2-mercaptoethanol
-
741195
Arabidopsis thaliana
(Z)-2-hydroxyethyl N-hydroxy-2-(4-hydroxyphenyl)ethanimidothioate + H2O
-
-
-
?
indole-3-acetaldoxime + O2 + glutathione + [reduced NADPH-hemoprotein reductase]
indole-3-acetaldoxime is the physiological substrate for isoform CYP83B1 but not for CYP83A1
741195
Arabidopsis thaliana
1-aci-nitro-2-indolyl-ethane + H2O + [oxidized NADPH-hemoprotein reductase]
-
-
-
?
additional information
isoform CYP83A1 catalyzes the initial conversion of aldoximes to thiohydroximates in the synthesis of glucosinolates not derived from tryptophan
741195
Arabidopsis thaliana
?
-
-
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cerevisiae
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
indole-3-acetaldoxime
pH not specified in the publication, temperature not specified in the publication
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(E)-4-hydroxyphenylacetaldoxime + O2 + [reduced NADPH-hemoprotein reductase]
-
741195
Arabidopsis thaliana
1-aci-nitro-2-(4-hydroxyphenyl)-ethane + H2O + [oxidized NADPH-hemoprotein reductase]
first step of reaction
-
-
?
(E)-phenylacetaldoxime + O2 + [reduced NADPH-hemoprotein reductase]
-
741195
Arabidopsis thaliana
1-aci-nitro-2-phenylethane + H2O + [oxidized NADPH-hemoprotein reductase]
first step of reaction
-
-
?
1-aci-nitro-2-(4-hydroxyphenyl)-ethane + 2-mercaptoethanol
-
741195
Arabidopsis thaliana
(Z)-2-hydroxyethyl N-hydroxy-2-(4-hydroxyphenyl)ethanimidothioate + H2O
-
-
-
?
indole-3-acetaldoxime + O2 + glutathione + [reduced NADPH-hemoprotein reductase]
indole-3-acetaldoxime is the physiological substrate for isoform CYP83B1 but not for CYP83A1
741195
Arabidopsis thaliana
1-aci-nitro-2-indolyl-ethane + H2O + [oxidized NADPH-hemoprotein reductase]
-
-
-
?
additional information
isoform CYP83A1 catalyzes the initial conversion of aldoximes to thiohydroximates in the synthesis of glucosinolates not derived from tryptophan
741195
Arabidopsis thaliana
?
-
-
-
-
General Information
General Information
Commentary
Organism
physiological function
ectopic overexpression of CYP83A1 rescues the rnt1-1 phenotype caused by knockout of CYP83B1 and showing a strong auxin excess phenotype
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
physiological function
ectopic overexpression of CYP83A1 rescues the rnt1-1 phenotype caused by knockout of CYP83B1 and showing a strong auxin excess phenotype
Arabidopsis thaliana
Other publictions for EC 1.14.14.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741219
Meenu
Genomic origin, expression dif ...
Brassica juncea
Planta
241
651-665
2015
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8
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1
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1
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1
1
1
1
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741068
Weis
CYP83A1 is required for metabo ...
Arabidopsis thaliana
New Phytol.
202
1310-1319
2014
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1
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5
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1
1
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740546
Zhu
Isolation and expression of gl ...
Brassica rapa subsp. chinensis
Int. J. Mol. Sci.
13
5832-5843
2012
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4
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3
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6
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741196
Naur
CYP83A1 and CYP83B1, two nonre ...
Arabidopsis thaliana
Plant Physiol.
133
63-72
2003
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-
1
-
-
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6
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5
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6
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6
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2
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6
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6
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6
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1
2
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6
6
741195
Bak
The involvement of two p450 en ...
Arabidopsis thaliana
Plant Physiol.
127
108-118
2001
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1
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1
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4
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5
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1
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1
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5
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1
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