BRENDA - Enzyme Database show
show all sequences of 1.14.14.44

Identification and characterization of CYP79D16 and CYP71AN24 catalyzing the first and second steps in L-phenylalanine-derived cyanogenic glycoside biosynthesis in the Japanese apricot, Prunus mume Sieb. et Zucc

Yamaguchi, T.; Yamamoto, K.; Asano, Y.; Plant Mol. Biol. 86, 215-223 (2014)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0039
-
(E)-phenylacetaldehyde oxime
pH 7.6, 30C
Prunus mume
0.007
-
4-hydroxyphenylacetaldoxime
pH 7.6, 30C
Prunus mume
0.0082
-
indole-3-acetaldoxime
pH 7.6, 30C
Prunus mume
0.0185
-
phenylacetonitrile
pH 7.6, 30C
Prunus mume
0.195
-
4-Hydroxyphenylacetonitrile
pH 7.6, 30C
Prunus mume
0.751
-
indole-3-acetonitrile
pH 7.6, 30C
Prunus mume
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(E)-phenylacetaldehyde oxime
Prunus mume
-
(Z)-phenylacetaldehyde oxime
-
-
?
(E)-phenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + O2
Prunus mume
-
(R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O
overall reaction
-
?
(Z)-phenylacetaldehyde oxime
Prunus mume
-
phenylacetonitrile + H2O
-
-
?
phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
Prunus mume
-
(R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Prunus mume
A0A068Q609
var. Sieb. et Zucc.
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Prunus mume
-
seedling
-
Prunus mume
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(E)-phenylacetaldehyde oxime
-
741183
Prunus mume
(Z)-phenylacetaldehyde oxime
-
-
-
?
(E)-phenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + O2
-
741183
Prunus mume
(R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O
overall reaction
-
-
?
(Z)-phenylacetaldehyde oxime
-
741183
Prunus mume
phenylacetonitrile + H2O
-
-
-
?
4-hydroxyphenylacetaldoxime + [reduced NADPH-hemoprotein reductase] + O2
-
741183
Prunus mume
hydroxy(4-hydroxyphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O
overall reaction
-
-
?
4-hydroxyphenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
-
741183
Prunus mume
hydroxy(4-hydroxyphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
indole-3-acetaldoxime + [reduced NADPH-hemoprotein reductase] + O2
reaction of EC 1.14.14.45
741183
Prunus mume
hydroxy(1H-indol-3-yl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O
overall reaction
-
-
?
indole-3-acetonitrile + [reduced NADPH-hemoprotein reductase] + O2
reaction of EC 1.14.14.45
741183
Prunus mume
hydroxy(1H-indol-3-yl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
additional information
enzyme converts aromatic aldoximes and nitriles to the corresponding hydroxynitriles. No substrates: aliphatic substrates 2-methylpropanal oxime and 2-methylpropanenitrile
741183
Prunus mume
?
-
-
-
-
phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
-
741183
Prunus mume
(R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.028
-
indole-3-acetaldoxime
pH 7.6, 30C
Prunus mume
0.1
-
indole-3-acetonitrile
pH 7.6, 30C
Prunus mume
0.4
-
phenylacetonitrile
pH 7.6, 30C
Prunus mume
0.57
-
4-Hydroxyphenylacetonitrile
pH 7.6, 30C
Prunus mume
0.62
-
4-hydroxyphenylacetaldoxime
pH 7.6, 30C
Prunus mume
0.77
-
(E)-phenylacetaldehyde oxime
pH 7.6, 30C
Prunus mume
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Prunus mume
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Prunus mume
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0039
-
(E)-phenylacetaldehyde oxime
pH 7.6, 30C
Prunus mume
0.007
-
4-hydroxyphenylacetaldoxime
pH 7.6, 30C
Prunus mume
0.0082
-
indole-3-acetaldoxime
pH 7.6, 30C
Prunus mume
0.0185
-
phenylacetonitrile
pH 7.6, 30C
Prunus mume
0.195
-
4-Hydroxyphenylacetonitrile
pH 7.6, 30C
Prunus mume
0.751
-
indole-3-acetonitrile
pH 7.6, 30C
Prunus mume
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(E)-phenylacetaldehyde oxime
Prunus mume
-
(Z)-phenylacetaldehyde oxime
-
-
?
(E)-phenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + O2
Prunus mume
-
(R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O
overall reaction
-
?
(Z)-phenylacetaldehyde oxime
Prunus mume
-
phenylacetonitrile + H2O
-
-
?
phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
Prunus mume
-
(R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Prunus mume
-
seedling
-
Prunus mume
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(E)-phenylacetaldehyde oxime
-
741183
Prunus mume
(Z)-phenylacetaldehyde oxime
-
-
-
?
(E)-phenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + O2
-
741183
Prunus mume
(R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O
overall reaction
-
-
?
(Z)-phenylacetaldehyde oxime
-
741183
Prunus mume
phenylacetonitrile + H2O
-
-
-
?
4-hydroxyphenylacetaldoxime + [reduced NADPH-hemoprotein reductase] + O2
-
741183
Prunus mume
hydroxy(4-hydroxyphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O
overall reaction
-
-
?
4-hydroxyphenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
-
741183
Prunus mume
hydroxy(4-hydroxyphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
indole-3-acetaldoxime + [reduced NADPH-hemoprotein reductase] + O2
reaction of EC 1.14.14.45
741183
Prunus mume
hydroxy(1H-indol-3-yl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O
overall reaction
-
-
?
indole-3-acetonitrile + [reduced NADPH-hemoprotein reductase] + O2
reaction of EC 1.14.14.45
741183
Prunus mume
hydroxy(1H-indol-3-yl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
additional information
enzyme converts aromatic aldoximes and nitriles to the corresponding hydroxynitriles. No substrates: aliphatic substrates 2-methylpropanal oxime and 2-methylpropanenitrile
741183
Prunus mume
?
-
-
-
-
phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
-
741183
Prunus mume
(R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.028
-
indole-3-acetaldoxime
pH 7.6, 30C
Prunus mume
0.1
-
indole-3-acetonitrile
pH 7.6, 30C
Prunus mume
0.4
-
phenylacetonitrile
pH 7.6, 30C
Prunus mume
0.57
-
4-Hydroxyphenylacetonitrile
pH 7.6, 30C
Prunus mume
0.62
-
4-hydroxyphenylacetaldoxime
pH 7.6, 30C
Prunus mume
0.77
-
(E)-phenylacetaldehyde oxime
pH 7.6, 30C
Prunus mume
Other publictions for EC 1.14.14.44
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741183
Yamaguchi
Identification and characteriz ...
Prunus mume
Plant Mol. Biol.
86
215-223
2014
-
-
-
-
-
-
-
6
-
-
-
4
-
4
-
-
-
-
-
2
-
-
9
-
-
-
-
6
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
6
-
-
-
4
-
-
-
-
-
2
-
-
9
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-