BRENDA - Enzyme Database show
show all sequences of 1.14.14.80

Expression and characterization of CYP4V2 as a fatty acid omega-hydroxylase

Nakano, M.; Kelly, E.J.; Rettie, A.E.; Drug Metab. Dispos. 37, 2119-2122 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed from pFastbacCYP4V2HIS vector in Escherichia coli DH10Bac. Hexahistidine-tagged CYP4V2 is expressed in Sf-9 cells after infection with the recombinant baculovirus
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
(E)-N'-(4-butyl-2-methylphenyl)-N-hydroxyformimidamide
HET0016
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.065
-
myristic acid
-
Homo sapiens
0.14
-
lauric acid
-
Homo sapiens
0.43
-
palmitic acid
-
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Homo sapiens
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
by Coomassie staining and by immunoblotting
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
eye
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
lauric acid + [reduced NADPH-hemoprotein reductase] + O2
-
703412
Homo sapiens
12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
octanoic acid is not detectably omega-hydroxylated by CYP4V2
703412
Homo sapiens
?
-
-
-
-
myristic acid + [reduced NADPH-hemoprotein reductase] + O2
CYP4V2 is a selective omega-hydroxylase of saturated, medium-chain fatty acids with relatively high catalytic efficiency toward myristic acid
703412
Homo sapiens
12-hydroxymyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2
-
703412
Homo sapiens
16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.000038
-
-
Homo sapiens
(E)-N'-(4-butyl-2-methylphenyl)-N-hydroxyformimidamide
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed from pFastbacCYP4V2HIS vector in Escherichia coli DH10Bac. Hexahistidine-tagged CYP4V2 is expressed in Sf-9 cells after infection with the recombinant baculovirus
Homo sapiens
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.000038
-
-
Homo sapiens
(E)-N'-(4-butyl-2-methylphenyl)-N-hydroxyformimidamide
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(E)-N'-(4-butyl-2-methylphenyl)-N-hydroxyformimidamide
HET0016
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.065
-
myristic acid
-
Homo sapiens
0.14
-
lauric acid
-
Homo sapiens
0.43
-
palmitic acid
-
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Homo sapiens
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
by Coomassie staining and by immunoblotting
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
eye
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
lauric acid + [reduced NADPH-hemoprotein reductase] + O2
-
703412
Homo sapiens
12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
octanoic acid is not detectably omega-hydroxylated by CYP4V2
703412
Homo sapiens
?
-
-
-
-
myristic acid + [reduced NADPH-hemoprotein reductase] + O2
CYP4V2 is a selective omega-hydroxylase of saturated, medium-chain fatty acids with relatively high catalytic efficiency toward myristic acid
703412
Homo sapiens
12-hydroxymyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2
-
703412
Homo sapiens
16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
defective omega-oxidation of ocular fatty acids/lipids secondary to mutations in the CYP4V2 gene may be a plausible mechanism underlying Bietti's crystalline dystrophy
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
physiological function
defective omega-oxidation of ocular fatty acids/lipids secondary to mutations in the CYP4V2 gene may be a plausible mechanism underlying Bietti's crystalline dystrophy
Homo sapiens
Other publictions for EC 1.14.14.80
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746320
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114
3181-3185
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1
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1
1
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746560
Uehara
Marmoset cytochrome P450 4A11 ...
Callithrix jacchus
Xenobiotica
47
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2017
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1
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1
1
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3
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4
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4
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1
3
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1
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3
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4
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744773
Han
Biochemical analysis of recom ...
Homo sapiens
Drug Metab. Pharmacokinet.
31
445-450
2016
1
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1
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3
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17
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1
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1
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3
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17
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1
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1
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2
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3
1
1
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17
1
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-
18
18
746057
Bjelica
Fatty acid omega-hydroxylases ...
Solanum tuberosum
Plant Cell Rep.
35
2435-2448
2016
-
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4
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1
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1
1
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744772
Saito
Functional characterization o ...
Homo sapiens
Drug Metab. Pharmacokinet.
30
119-122
2015
-
-
1
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9
-
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7
-
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1
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1
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1
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1
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9
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7
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1
-
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1
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-
733174
Huang
Expression and characterizatio ...
Starmerella bombicola
Appl. Environ. Microbiol.
80
766-776
2014
-
-
-
-
-
-
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3
-
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6
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14
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1
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1
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3
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14
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1
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1
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1
1
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744097
Huang
Expression and characterizati ...
Starmerella bombicola, Starmerella bombicola ATCC 22214
Appl. Environ. Microbiol.
80
766-776
2014
-
-
1
-
-
-
-
3
1
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28
-
9
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1
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36
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2
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1
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2
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3
6
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3
3
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28
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3
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36
-
3
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3
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2
6
-
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744323
Kim
Kinetic analysis of lauric ac ...
Homo sapiens
Biochemistry
53
6161-6172
2014
1
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2
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1
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1
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703412
Nakano
Expression and characterizatio ...
Homo sapiens
Drug Metab. Dispos.
37
2119-2122
2009
-
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1
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-
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1
3
1
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1
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1
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1
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4
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1
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1
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1
1
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3
1
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1
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1
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4
-
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-
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1
1
-
-
-
706321
Dobritsa
CYP704B1 is a long-chain fatty ...
Arabidopsis thaliana
Plant Physiol.
151
574-589
2009
-
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1
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-
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-
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3
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2
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7
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1
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2
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7
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1
1
1
1
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-
733891
Van Bogaert
Importance of the cytochrome P ...
Starmerella bombicola
FEMS Yeast Res.
9
87-94
2009
-
-
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3
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1
1
1
1
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734431
Gatica
P450 CYP2C epoxygenase and CYP ...
Rattus norvegicus
J. Lipid Res.
48
924-934
2007
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1
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1
1
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676666
Benveniste
Evolutionary relationship and ...
Arabidopsis thaliana
Plant Sci.
170
326-338
2006
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1
1
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1
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4
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734950
Duan
Differential expression and ev ...
Arabidopsis thaliana
Plant Physiol.
137
1067-1081
2005
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8
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7
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5
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15
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5
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2
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8
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285382
Hoch
Structural determination of th ...
Homo sapiens, Rattus norvegicus
Arch. Biochem. Biophys.
373
63-71
2000
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2
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1
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13
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8
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2
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25
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21
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5
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2
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14
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5
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25
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24
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733243
Benveniste
CYP86A1 from Arabidopsis thali ...
Arabidopsis thaliana
Biochem. Biophys. Res. Commun.
243
688-693
1998
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734106
Hiratsuka
Sex and strain differences in ...
Mus musculus
J. Biochem.
119
340-345
1996
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734105
Kawashima
Purification and cDNA cloning ...
Homo sapiens
J. Biochem.
116
74-80
1994
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