BRENDA - Enzyme Database show
show all sequences of 1.14.14.80

CYP704B1 is a long-chain fatty acid omega-hydroxylase essential for sporopollenin synthesis in pollen of Arabidopsis

Dobritsa, A.A.; Shrestha, J.; Morant, M.; Pinot, F.; Matsuno, M.; Swanson, R.; Moller, B.L.; Preuss, D.; Plant Physiol. 151, 574-589 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in yeast WAT11 cells
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9C788
isoform CYP704B1
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
anther
CYP704B1 is expressed in the developing anthers
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
9,10-epoxystearic acid + [reduced NADPH-hemoprotein reductase] + O2
-
706321
Arabidopsis thaliana
18-hydroxy-9,10-epoxystearic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
linoleic acid + [reduced NADPH-hemoprotein reductase] + O2
-
706321
Arabidopsis thaliana
18-hydroxylinoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
linolenic acid + [reduced NADPH-hemoprotein reductase] + O2
best substrate
706321
Arabidopsis thaliana
18-hydroxylinolenic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
no substrate: 12-hydroxylauric acid, lauric acid, cinnamate, p-coumarate, ferulate, sinapate, and p-coumaroyl shikimate
706321
Arabidopsis thaliana
?
-
-
-
-
oleic acid + [reduced NADPH-hemoprotein reductase] + O2
28% yield
706321
Arabidopsis thaliana
18-hydroxyoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2
21% yield
706321
Arabidopsis thaliana
16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
palmitoleic acid + [reduced NADPH-hemoprotein reductase] + O2
44% yield
706321
Arabidopsis thaliana
18-hydroxypalmitoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in yeast WAT11 cells
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
anther
CYP704B1 is expressed in the developing anthers
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
9,10-epoxystearic acid + [reduced NADPH-hemoprotein reductase] + O2
-
706321
Arabidopsis thaliana
18-hydroxy-9,10-epoxystearic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
linoleic acid + [reduced NADPH-hemoprotein reductase] + O2
-
706321
Arabidopsis thaliana
18-hydroxylinoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
linolenic acid + [reduced NADPH-hemoprotein reductase] + O2
best substrate
706321
Arabidopsis thaliana
18-hydroxylinolenic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
no substrate: 12-hydroxylauric acid, lauric acid, cinnamate, p-coumarate, ferulate, sinapate, and p-coumaroyl shikimate
706321
Arabidopsis thaliana
?
-
-
-
-
oleic acid + [reduced NADPH-hemoprotein reductase] + O2
28% yield
706321
Arabidopsis thaliana
18-hydroxyoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2
21% yield
706321
Arabidopsis thaliana
16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
palmitoleic acid + [reduced NADPH-hemoprotein reductase] + O2
44% yield
706321
Arabidopsis thaliana
18-hydroxypalmitoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Expression
Organism
Commentary
Expression
Arabidopsis thaliana
CYP704B1 is highly coexpressed with CYP703A2, a cytochrome P450 involved in exine production
additional information
General Information
General Information
Commentary
Organism
physiological function
mutations in CYP704B1 result in impaired pollen walls that lack a normal exine layer and exhibit a characteristic striped surface, termed zebra phenotyp. Mutations in three genes, CYP704B1, CYP703A2, and another exine gene, MALE STERILITY2 result in pollen with remarkably similar zebra phenotypes, distinct from those of other known exine mutants. The double and triple mutant combinations do not result in the appearance of novel phenotypes or enhancement of single mutant phenotypes
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
physiological function
mutations in CYP704B1 result in impaired pollen walls that lack a normal exine layer and exhibit a characteristic striped surface, termed zebra phenotyp. Mutations in three genes, CYP704B1, CYP703A2, and another exine gene, MALE STERILITY2 result in pollen with remarkably similar zebra phenotypes, distinct from those of other known exine mutants. The double and triple mutant combinations do not result in the appearance of novel phenotypes or enhancement of single mutant phenotypes
Arabidopsis thaliana
Expression (protein specific)
Organism
Commentary
Expression
Arabidopsis thaliana
CYP704B1 is highly coexpressed with CYP703A2, a cytochrome P450 involved in exine production
additional information
Other publictions for EC 1.14.14.80
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746320
Zhang
Ablation of cytochrome P450 o ...
Mus musculus
Proc. Natl. Acad. Sci. USA
114
3181-3185
2017
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1
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1
1
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746560
Uehara
Marmoset cytochrome P450 4A11 ...
Callithrix jacchus
Xenobiotica
47
553-561
2017
-
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1
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1
1
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3
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4
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3
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4
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3
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1
3
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1
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3
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4
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744773
Han
Biochemical analysis of recom ...
Homo sapiens
Drug Metab. Pharmacokinet.
31
445-450
2016
1
-
1
-
3
-
-
17
-
1
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3
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1
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1
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2
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3
1
1
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-
17
1
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3
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1
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1
3
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3
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-
-
17
-
1
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3
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1
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2
-
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3
1
1
-
-
17
1
-
-
-
-
-
-
-
18
18
746057
Bjelica
Fatty acid omega-hydroxylases ...
Solanum tuberosum
Plant Cell Rep.
35
2435-2448
2016
-
-
-
-
-
-
-
-
-
-
-
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-
4
-
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1
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1
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1
1
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-
744772
Saito
Functional characterization o ...
Homo sapiens
Drug Metab. Pharmacokinet.
30
119-122
2015
-
-
1
-
9
-
-
7
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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-
1
-
-
9
-
-
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-
7
-
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-
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-
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-
1
-
-
1
-
-
-
-
-
-
-
-
-
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-
-
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-
733174
Huang
Expression and characterizatio ...
Starmerella bombicola
Appl. Environ. Microbiol.
80
766-776
2014
-
-
-
-
-
-
-
3
-
-
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6
-
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14
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1
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1
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3
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14
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1
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1
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1
1
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744097
Huang
Expression and characterizati ...
Starmerella bombicola, Starmerella bombicola ATCC 22214
Appl. Environ. Microbiol.
80
766-776
2014
-
-
1
-
-
-
-
3
1
-
-
28
-
9
-
-
1
-
-
-
-
-
36
-
2
-
-
-
1
-
-
2
-
-
-
-
-
3
6
-
-
-
-
-
-
3
3
-
-
28
-
-
-
3
-
-
-
-
36
-
3
-
-
-
3
-
-
-
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2
6
-
-
-
744323
Kim
Kinetic analysis of lauric ac ...
Homo sapiens
Biochemistry
53
6161-6172
2014
1
-
-
-
-
-
-
-
-
-
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2
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2
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2
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1
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2
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703412
Nakano
Expression and characterizatio ...
Homo sapiens
Drug Metab. Dispos.
37
2119-2122
2009
-
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1
-
-
-
1
3
1
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1
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1
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1
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4
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1
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1
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1
1
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3
1
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1
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-
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1
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4
-
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-
-
-
-
-
-
-
-
1
1
-
-
-
706321
Dobritsa
CYP704B1 is a long-chain fatty ...
Arabidopsis thaliana
Plant Physiol.
151
574-589
2009
-
-
1
-
-
-
-
-
-
-
-
-
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3
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2
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7
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1
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2
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7
-
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-
-
-
-
-
1
1
1
1
-
-
733891
Van Bogaert
Importance of the cytochrome P ...
Starmerella bombicola
FEMS Yeast Res.
9
87-94
2009
-
-
-
-
-
-
-
-
-
-
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-
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3
-
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1
1
1
1
-
-
734431
Gatica
P450 CYP2C epoxygenase and CYP ...
Rattus norvegicus
J. Lipid Res.
48
924-934
2007
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1
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1
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1
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1
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1
1
1
1
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-
676666
Benveniste
Evolutionary relationship and ...
Arabidopsis thaliana
Plant Sci.
170
326-338
2006
-
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1
1
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1
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4
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1
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1
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4
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734950
Duan
Differential expression and ev ...
Arabidopsis thaliana
Plant Physiol.
137
1067-1081
2005
-
-
-
-
-
-
-
-
-
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8
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7
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5
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15
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5
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2
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8
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285382
Hoch
Structural determination of th ...
Homo sapiens, Rattus norvegicus
Arch. Biochem. Biophys.
373
63-71
2000
-
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2
-
1
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13
-
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8
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2
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25
-
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21
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5
-
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2
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14
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5
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25
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24
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733243
Benveniste
CYP86A1 from Arabidopsis thali ...
Arabidopsis thaliana
Biochem. Biophys. Res. Commun.
243
688-693
1998
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1
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1
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6
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1
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6
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1
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734106
Hiratsuka
Sex and strain differences in ...
Mus musculus
J. Biochem.
119
340-345
1996
-
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3
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1
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734105
Kawashima
Purification and cDNA cloning ...
Homo sapiens
J. Biochem.
116
74-80
1994
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1
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1
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1
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8
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1
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