BRENDA - Enzyme Database show
show all sequences of 1.14.14.80

Expression and characterization of CYP52 genes involved in the biosynthesis of sophorolipid and alkane metabolism from Starmerella bombicola

Huang, F.C.; Peter, A.; Schwab, W.; Appl. Environ. Microbiol. 80, 766-776 (2014)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
oleic acid
pH 7.0, 30C
Starmerella bombicola
0.054
-
arachidonic acid
pH 7.0, 30C
Starmerella bombicola
0.068
-
linoleic acid
pH 7.0, 30C
Starmerella bombicola
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Starmerella bombicola
B8QHP1
-
-
Starmerella bombicola
B8QHP3
-
-
Starmerella bombicola
B8QHP5
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-linolenic acid + [reduced NADPH-hemoprotein reductase] + O2
-
733174
Starmerella bombicola
18-hydroxylinolenic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2
-
733174
Starmerella bombicola
20-hydroxyarachidonic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
linoleic acid + [reduced NADPH-hemoprotein reductase] + O2
-
733174
Starmerella bombicola
18-hydroxylinoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
isoform CYP52M1 oxidizes C16 to C20 fatty acids preferentially. It converts oleic acid (C18:1) more efficiently than stearic acid (C18:0) and linoleic acid (C18:2) and much more effectively than alpha-linolenic acid (C18:3). No products are detected when C10 to C12 fatty acids are used as the substrates. CYP52M1 hydroxylates fatty acids at their omega- and omega-1 positions. No activity is detected toward short-chain fatty acids (C10 to C14), cis-11-eicosenoic acid (C20:1), and C16 to C18 alkanes
733174
Starmerella bombicola
?
-
-
-
-
additional information
isoform CYP52N1 oxidizes C14 to C20 saturated and unsaturated fatty acids and preferentially oxidizes palmitic acid, oleic acid, and linoleic acid. It only catalyzes omega-hydroxylation of fatty acids
733174
Starmerella bombicola
?
-
-
-
-
additional information
isoform CYP52N1 shows minor omega-hydroxylation activity against myristic acid, palmitic acid, palmitoleic acid, and oleic acid
733174
Starmerella bombicola
?
-
-
-
-
myristic acid + [reduced NADPH-hemoprotein reductase] + O2
-
733174
Starmerella bombicola
14-hydroxymyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
oleic acid + [reduced NADPH-hemoprotein reductase] + O2
-
733174
Starmerella bombicola
18-hydroxyoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2
-
733174
Starmerella bombicola
16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
palmitoleic acid + [reduced NADPH-hemoprotein reductase] + O2
-
733174
Starmerella bombicola
18-hydroxypalmitoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
30
-
Starmerella bombicola
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Starmerella bombicola
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
oleic acid
pH 7.0, 30C
Starmerella bombicola
0.054
-
arachidonic acid
pH 7.0, 30C
Starmerella bombicola
0.068
-
linoleic acid
pH 7.0, 30C
Starmerella bombicola
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-linolenic acid + [reduced NADPH-hemoprotein reductase] + O2
-
733174
Starmerella bombicola
18-hydroxylinolenic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2
-
733174
Starmerella bombicola
20-hydroxyarachidonic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
linoleic acid + [reduced NADPH-hemoprotein reductase] + O2
-
733174
Starmerella bombicola
18-hydroxylinoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
isoform CYP52M1 oxidizes C16 to C20 fatty acids preferentially. It converts oleic acid (C18:1) more efficiently than stearic acid (C18:0) and linoleic acid (C18:2) and much more effectively than alpha-linolenic acid (C18:3). No products are detected when C10 to C12 fatty acids are used as the substrates. CYP52M1 hydroxylates fatty acids at their omega- and omega-1 positions. No activity is detected toward short-chain fatty acids (C10 to C14), cis-11-eicosenoic acid (C20:1), and C16 to C18 alkanes
733174
Starmerella bombicola
?
-
-
-
-
additional information
isoform CYP52N1 oxidizes C14 to C20 saturated and unsaturated fatty acids and preferentially oxidizes palmitic acid, oleic acid, and linoleic acid. It only catalyzes omega-hydroxylation of fatty acids
733174
Starmerella bombicola
?
-
-
-
-
additional information
isoform CYP52N1 shows minor omega-hydroxylation activity against myristic acid, palmitic acid, palmitoleic acid, and oleic acid
733174
Starmerella bombicola
?
-
-
-
-
myristic acid + [reduced NADPH-hemoprotein reductase] + O2
-
733174
Starmerella bombicola
14-hydroxymyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
oleic acid + [reduced NADPH-hemoprotein reductase] + O2
-
733174
Starmerella bombicola
18-hydroxyoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2
-
733174
Starmerella bombicola
16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
palmitoleic acid + [reduced NADPH-hemoprotein reductase] + O2
-
733174
Starmerella bombicola
18-hydroxypalmitoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
30
-
Starmerella bombicola
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Starmerella bombicola
General Information
General Information
Commentary
Organism
physiological function
isoform CYP52M1 is involved in the biosynthesis of sophorolipid
Starmerella bombicola
General Information (protein specific)
General Information
Commentary
Organism
physiological function
isoform CYP52M1 is involved in the biosynthesis of sophorolipid
Starmerella bombicola
Other publictions for EC 1.14.14.80
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746320
Zhang
Ablation of cytochrome P450 o ...
Mus musculus
Proc. Natl. Acad. Sci. USA
114
3181-3185
2017
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1
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1
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1
1
-
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746560
Uehara
Marmoset cytochrome P450 4A11 ...
Callithrix jacchus
Xenobiotica
47
553-561
2017
-
-
1
-
-
-
-
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1
1
-
3
-
4
-
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3
-
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4
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3
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1
3
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1
1
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3
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3
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4
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744773
Han
Biochemical analysis of recom ...
Homo sapiens
Drug Metab. Pharmacokinet.
31
445-450
2016
1
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1
-
3
-
-
17
-
1
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3
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1
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1
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2
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3
1
1
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17
1
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3
-
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1
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1
3
-
3
-
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-
17
-
1
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3
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-
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1
-
2
-
-
3
1
1
-
-
17
1
-
-
-
-
-
-
-
18
18
746057
Bjelica
Fatty acid omega-hydroxylases ...
Solanum tuberosum
Plant Cell Rep.
35
2435-2448
2016
-
-
-
-
-
-
-
-
-
-
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-
-
4
-
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1
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1
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-
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1
1
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-
744772
Saito
Functional characterization o ...
Homo sapiens
Drug Metab. Pharmacokinet.
30
119-122
2015
-
-
1
-
9
-
-
7
-
-
-
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1
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1
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1
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1
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9
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7
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1
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1
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-
-
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733174
Huang
Expression and characterizatio ...
Starmerella bombicola
Appl. Environ. Microbiol.
80
766-776
2014
-
-
-
-
-
-
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3
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6
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14
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1
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1
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3
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14
-
1
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-
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1
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1
1
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-
744097
Huang
Expression and characterizati ...
Starmerella bombicola, Starmerella bombicola ATCC 22214
Appl. Environ. Microbiol.
80
766-776
2014
-
-
1
-
-
-
-
3
1
-
-
28
-
9
-
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1
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36
-
2
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1
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2
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3
6
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-
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3
3
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28
-
-
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3
-
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36
-
3
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-
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3
-
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2
6
-
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-
744323
Kim
Kinetic analysis of lauric ac ...
Homo sapiens
Biochemistry
53
6161-6172
2014
1
-
-
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2
-
2
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1
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1
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1
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2
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703412
Nakano
Expression and characterizatio ...
Homo sapiens
Drug Metab. Dispos.
37
2119-2122
2009
-
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1
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-
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1
3
1
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1
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1
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1
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4
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1
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1
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1
1
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3
1
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1
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1
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4
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1
1
-
-
-
706321
Dobritsa
CYP704B1 is a long-chain fatty ...
Arabidopsis thaliana
Plant Physiol.
151
574-589
2009
-
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1
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3
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2
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7
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2
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7
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1
1
1
1
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733891
Van Bogaert
Importance of the cytochrome P ...
Starmerella bombicola
FEMS Yeast Res.
9
87-94
2009
-
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3
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1
1
1
1
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734431
Gatica
P450 CYP2C epoxygenase and CYP ...
Rattus norvegicus
J. Lipid Res.
48
924-934
2007
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1
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1
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1
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1
1
1
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676666
Benveniste
Evolutionary relationship and ...
Arabidopsis thaliana
Plant Sci.
170
326-338
2006
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1
1
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4
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1
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734950
Duan
Differential expression and ev ...
Arabidopsis thaliana
Plant Physiol.
137
1067-1081
2005
-
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8
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7
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15
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5
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2
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8
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285382
Hoch
Structural determination of th ...
Homo sapiens, Rattus norvegicus
Arch. Biochem. Biophys.
373
63-71
2000
-
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2
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1
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13
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8
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2
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25
-
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21
-
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5
-
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2
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14
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5
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25
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24
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733243
Benveniste
CYP86A1 from Arabidopsis thali ...
Arabidopsis thaliana
Biochem. Biophys. Res. Commun.
243
688-693
1998
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1
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6
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1
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734106
Hiratsuka
Sex and strain differences in ...
Mus musculus
J. Biochem.
119
340-345
1996
-
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3
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1
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1
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734105
Kawashima
Purification and cDNA cloning ...
Homo sapiens
J. Biochem.
116
74-80
1994
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1
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1
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