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Literature summary for 1.14.14.92 extracted from

  • Van Den Brink, J.M.; Van Den Hondel, C.A.M.J.J.; Van Gorcom, R.F.M.
    Optimization of the benzoate-inducible benzoate p-hydroxylase cytochrome P450 enzyme system in Aspergillus niger (1996), Appl. Microbiol. Biotechnol., 46, 360-364.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
coexpression of benzoate 4-hydroxylase and cytochrome P450 reductase in Aspergillus niger increases enzyme activity Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger N204
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger 4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
benzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger N204 4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
 benzoate reconstituted enzyme system consisting of benzoate 4-hydroxylase, cytochrome P450 reductase, NADPH and dilauroylphosphatidylcholine Aspergillus niger