BRENDA - Enzyme Database show
show all sequences of 1.14.14.92

Heterologous expression of the benzoate para-hydroxylase encoding gene (CYP53B1) from Rhodotorula minuta by Yarrowia lipolytica

Shiningavamwe, A.; Obiero, G.; Albertyn, J.; Nicaud, J.M.; Smit, M.; Appl. Microbiol. Biotechnol. 72, 323-329 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene CYP53B1, functional overexpression in Yarrowia lipolytica strain UOFS Y-2366, multiple copies of CYP53B1 cDNA cloned under control of POX2 promoter, construction of several clones, feeding of stearic acid to the recombinant yeast leads to high accumulation of 4-hydroxybenzoate
Cystobasidium minutum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzoic acid + [reduced NADPH-hemoprotein reductase] + O2
Cystobasidium minutum
-
4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
benzoic acid + [reduced NADPH-hemoprotein reductase] + O2
Cystobasidium minutum CBS 2177
-
4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cystobasidium minutum
-
gene CYP53B1
-
Cystobasidium minutum CBS 2177
-
gene CYP53B1
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
recombinant enzyme expressed in Yarrowia lipolytica clones, overview
Cystobasidium minutum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
671533
Cystobasidium minutum
4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
benzoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
671533
Cystobasidium minutum CBS 2177
4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
gene CYP53B1, functional overexpression in Yarrowia lipolytica strain UOFS Y-2366, multiple copies of CYP53B1 cDNA cloned under control of POX2 promoter, construction of several clones, feeding of stearic acid to the recombinant yeast leads to high accumulation of 4-hydroxybenzoate
Cystobasidium minutum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzoic acid + [reduced NADPH-hemoprotein reductase] + O2
Cystobasidium minutum
-
4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
benzoic acid + [reduced NADPH-hemoprotein reductase] + O2
Cystobasidium minutum CBS 2177
-
4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
recombinant enzyme expressed in Yarrowia lipolytica clones, overview
Cystobasidium minutum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
671533
Cystobasidium minutum
4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
benzoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
671533
Cystobasidium minutum CBS 2177
4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Other publictions for EC 1.14.14.92
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741716
Antunes
A benzoate-activated promoter ...
Aspergillus niger
Appl. Microbiol. Biotechnol.
100
5479-5489
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
742764
Korosec
Antifungal activity of cinnam ...
Curvularia lunata
J. Appl. Microbiol.
116
955-966
2014
-
2
1
-
-
-
1
-
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
725603
Berne
Virtual screening yields inhib ...
Curvularia lunata
J. Chem. Inf. Model.
52
3053-3063
2012
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686803
Kim
Molecular cloning and function ...
Chromohalobacter sp. HS2
FEMS Microbiol. Lett.
280
235-241
2008
-
-
1
-
-
-
-
-
-
1
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671533
Shiningavamwe
Heterologous expression of the ...
Cystobasidium minutum, Cystobasidium minutum CBS 2177
Appl. Microbiol. Biotechnol.
72
323-329
2006
-
-
1
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671704
Matsuzaki
Molecular characterization of ...
Phanerochaete chrysosporium
Biochem. Biophys. Res. Commun.
334
1184-1190
2005
1
-
1
-
-
-
-
-
1
1
1
1
-
1
-
-
-
-
-
-
-
-
5
1
1
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
1
1
1
-
-
-
-
-
-
-
-
5
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
440261
Faber
Purification and characterizat ...
Aspergillus niger
Arch. Biochem. Biophys.
394
245-254
2001
-
-
-
-
-
-
-
9
-
-
-
-
-
1
-
-
1
-
-
-
-
-
12
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
1
-
-
-
-
12
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
440259
Fukuda
Cytochrome P450rm from Rhodoto ...
Cystobasidium minutum
J. Biochem.
119
314-318
1996
-
-
-
-
-
-
3
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
3
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
440260
Van Den Brink
Optimization of the benzoate-i ...
Aspergillus niger, Aspergillus niger N204
Appl. Microbiol. Biotechnol.
46
360-364
1996
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
440254
McNamee
Properties of a membrane-assoc ...
Rhodotorula graminis
Biochem. Biophys. Res. Commun.
129
485-492
1985
-
-
1
-
-
-
15
2
1
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
15
-
2
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
4804
Durham
Initial reactions involved in ...
Rhodotorula graminis
J. Bacteriol.
160
778-780
1984
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
440258
Shailubhai
-
Degradation of chlorinated der ...
Aspergillus niger
FEMS Microbiol. Lett.
18
279-282
1983
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
440255
Reddy
Purification and properties of ...
Pseudomonas sp.
Arch. Biochem. Biophys.
177
488-498
1976
3
-
-
-
-
2
9
3
-
1
1
-
-
1
-
-
1
-
-
-
1
2
3
-
1
-
-
-
1
1
-
1
1
-
-
3
-
-
1
-
-
2
-
9
1
3
-
1
1
-
-
-
-
1
-
-
1
2
3
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
440256
Reddy
Purification, properties and i ...
Aspergillus niger, Aspergillus niger UBC 814
Biochim. Biophys. Acta
384
46-57
1975
1
-
-
-
-
3
13
2
-
1
-
-
-
6
-
-
1
-
-
2
1
1
2
-
1
1
-
-
1
1
1
1
-
-
-
1
-
-
1
-
-
3
-
13
-
2
-
1
-
-
-
-
-
1
-
2
1
1
2
-
1
1
-
-
1
1
1
-
-
-
-
-
-
-