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Literature summary for 1.14.14.B11 extracted from

  • Gavilano, L.B.; Coleman, N.P.; Bowen, S.W.; Siminszky, B.
    Functional analysis of nicotine demethylase genes reveals insights into the evolution of modern tobacco (2007), J. Biol. Chem., 282, 249-256.
    View publication on PubMed
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Protein Variants

Protein Variants Comment Organism
C330W isoform CYP82E3 variant, active Nicotiana tomentosiformis
K269N isoform CYP82E3 variant, no catalytic activity Nicotiana tomentosiformis
K269N/I274Null isoform CYP82E3 variant, no catalytic activity Nicotiana tomentosiformis
K269N/I274Null/T279A isoform CYP82E3 variant, no catalytic activity Nicotiana tomentosiformis
additional information wild-type isoform CYP82E3 shares 95% predicted amino acid sequence identity with isoform CYP82E4 but conferrs no activity when expressed in yeast or tobacco. Series of domain-swapping experiments between isoforms CYP82E3 and CYP82E4 to isentify regions and amino acids required for catalytic activity Nicotiana tabacum
additional information wild-type isoform CYP82E3 shares 95% predicted amino acid sequence identity with isoform CYP82E4 but confers no activity when expressed in yeast or tobacco. Series of domain-swapping experiments between isoforms CYP82E3 and CYP82E4 to identify regions and amino acids required for catalytic activity Nicotiana tomentosiformis
additional information wild-type isoform CYP82E3 shares 95% predicted amino acid sequence identity with isoform CYP82E4 but confers no activity when expressed in yeast or tobacco. Series of domain-swapping experiments between isoforms CYP82E3 and CYP82E4 to isentify regions and amino acids required for catalytic activity Nicotiana tomentosiformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0091
-
 Nicotine 23°C, pH 7.1 Nicotiana tomentosiformis
0.0481
-
 Nicotine 23°C, pH 7.1 Nicotiana tomentosiformis

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
 isoform CYP82E3
-
Nicotiana tomentosiformis A1YJE2 isoform CYP82E3
-
Nicotiana tomentosiformis A1YJE3 isoform CYP82E4
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf high expression in green leaf Nicotiana tomentosiformis
-
leaf high induced during leaf senescence Nicotiana tomentosiformis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotine + [reduced NADPH-hemoprotein reductase] + O2
-
 Nicotiana tomentosiformis nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?