Cloned (Comment) | Organism |
---|---|
D121-AA8 cDNA transformed into yeast cells | Nicotiana tabacum |
expression in yeast | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-aminobenzotriazole | at 1 mM inhibits by 17% | Nicotiana tabacum | |
Chloropromazine | 1 mM, 87% inhibition of recombinant nicotine demethylase activity in yeast microsomes | Nicotiana tabacum | |
chlorpromazine | at 1 mM inhibits by 87% | Nicotiana tabacum | |
CO | partial reverse activity after light exposure | Nicotiana tabacum | |
cytochrome c | 1 mM, 78% inhibition of recombinant nicotine demethylase activity in yeast microsomes | Nicotiana tabacum | |
oxidized cytochrome c | at 0.1 mM inhibits by 78% | Nicotiana tabacum | |
Piperonylbutoxide | at 1 mM inhibits by 42% | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0039 | - |
Nicotine | - |
Nicotiana tabacum | |
0.0039 | - |
Nicotine | at 25°C, pH 6.25 | Nicotiana tabacum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | putative N-terminal hydrophobic trans-membrane domain | Nicotiana tabacum | 16020 | - |
microsome | - |
Nicotiana tabacum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | cysteine-containing heme-binding region | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotine + NADPH + O2 + H+ | Nicotiana tabacum | demethylates nicotine to form nornicotine, a precursor to the nitrosamine N'-nitrosonornicotine | nornicotine + formaldehyde + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | Q38Q87 | - |
- |
Nicotiana tabacum | Q38Q87 | var. 4407 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nicotiana tabacum | - |
leaf | induced in leaves treated with the growth regulator ethylene | Nicotiana tabacum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotine + NADPH + O2 + H+ | - |
Nicotiana tabacum | nornicotine + formaldehyde + NADP+ + H2O | - |
? | |
nicotine + NADPH + O2 + H+ | demethylates nicotine to form nornicotine, a precursor to the nitrosamine N'-nitrosonornicotine | Nicotiana tabacum | nornicotine + formaldehyde + NADP+ + H2O | - |
? | |
nicotine + NADPH + O2 + H+ | six putative substrate recognition sites | Nicotiana tabacum | nornicotine + formaldehyde + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP82E4 | - |
Nicotiana tabacum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Nicotiana tabacum | |
NADPH | - |
Nicotiana tabacum | |
NADPH | is required for full activity | Nicotiana tabacum |
Organism | Comment | Expression |
---|---|---|
Nicotiana tabacum | ethylene induces microsomal demethylase activity in leaves | up |
General Information | Comment | Organism |
---|---|---|
physiological function | only microsomes from yeast expressing the D121-AA8 cDNA exhibit significant nicotine demethylase activity | Nicotiana tabacum |