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Literature summary for 1.14.15.39 extracted from

  • Moody, S.C.; Zhao, B.; Lei, L.; Nelson, D.R.; Mullins, J.G.; Waterman, M.R.; Kelly, S.L.; Lamb, D.C.
    Investigating conservation of the albaflavenone biosynthetic pathway and CYP170 bifunctionality in Streptomycetes (2012), FEBS J., 279, 1640-1649.
    View publication on PubMed
Search for more literature for 1.14.15.39

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Streptomyces coelicolor

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Streptomyces coelicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 55000, SDS-PAGE Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-epi-isozizaene + 4 reduced [2Fe-2S] ferredoxin + 4 H+ + 2 O2 Streptomyces coelicolor
-
 albaflavenone + 4 oxidized [2Fe-2S] ferredoxin + 3 H2O
-
 ?
(+)-epi-isozizaene + 4 reduced [2Fe-2S] ferredoxin + 4 H+ + 2 O2 Streptomyces coelicolor A3(2) M145
-
 albaflavenone + 4 oxidized [2Fe-2S] ferredoxin + 3 H2O
-
 ?
additional information Streptomyces coelicolor the bifunctional enzyme is able to also generate farnesene isomers from farnesyl diphosphate: (E)-beta-farnesene (61%), (3E,6E)-alpha-farnesene (26%), (3Z,6E)-alpha-farnesene (6.8%), nerolidol (4.9%), and farnesol(1.8%) ?
-
 ?
additional information Streptomyces coelicolor A3(2) M145 the bifunctional enzyme is able to also generate farnesene isomers from farnesyl diphosphate: (E)-beta-farnesene (61%), (3E,6E)-alpha-farnesene (26%), (3Z,6E)-alpha-farnesene (6.8%), nerolidol (4.9%), and farnesol(1.8%) ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
-
-
Streptomyces coelicolor A3(2) M145
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-affinity column chromatography Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-epi-isozizaene + 4 reduced [2Fe-2S] ferredoxin + 4 H+ + 2 O2
-
 Streptomyces coelicolor albaflavenone + 4 oxidized [2Fe-2S] ferredoxin + 3 H2O
-
 ?
(+)-epi-isozizaene + 4 reduced [2Fe-2S] ferredoxin + 4 H+ + 2 O2
-
 Streptomyces coelicolor A3(2) M145 albaflavenone + 4 oxidized [2Fe-2S] ferredoxin + 3 H2O
-
 ?
additional information the bifunctional enzyme is able to also generate farnesene isomers from farnesyl diphosphate: (E)-beta-farnesene (61%), (3E,6E)-alpha-farnesene (26%), (3Z,6E)-alpha-farnesene (6.8%), nerolidol (4.9%), and farnesol(1.8%) Streptomyces coelicolor ?
-
 ?
additional information the bifunctional enzyme is able to also generate farnesene isomers from farnesyl diphosphate: (E)-beta-farnesene (61%), (3E,6E)-alpha-farnesene (26%), (3Z,6E)-alpha-farnesene (6.8%), nerolidol (4.9%), and farnesol(1.8%) Streptomyces coelicolor A3(2) M145 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Streptomyces coelicolor

Synonyms

Synonyms Comment Organism
CYP170A1
-
 Streptomyces coelicolor

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Streptomyces coelicolor