Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | - |
Bos taurus | 5743 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
2,4,6,8 * 48000, SDS-PAGE, corpus luteum. x * 46000, x * 60000, x * 53000, x * 52000-53000 adrenal mitochondria | Bos taurus |
48000 | - |
2,4,6,8 * 48000, SDS-PAGE, corpus luteum. x * 46000, x * 60000, x * 53000, x * 52000-53000 adrenal mitochondria | Bos taurus |
53000 | - |
2,4,6,8 * 48000, SDS-PAGE, corpus luteum. x * 46000, x * 60000, x * 53000, x * 52000-53000 adrenal mitochondria | Bos taurus |
60000 | - |
2,4,6,8 * 48000, SDS-PAGE, corpus luteum. x * 46000, x * 60000, x * 53000, x * 52000-53000 adrenal mitochondria | Bos taurus |
97000 | - |
sedimentation equilibrium | Bos taurus |
100000 | - |
native-PAGE, incomplete enzyme or molecular environmental conditions not optimal | Bos taurus |
200000 | - |
native-PAGE, lowest single unit with high enzymatic activity | Bos taurus |
220000 | - |
corpus luteum | Bos taurus |
225000 | - |
sedimentation equilibrium | Bos taurus |
400000 | - |
native-PAGE | Bos taurus |
415000 | - |
sedimentation equilibrium | Bos taurus |
850000 | - |
adrenal mitochondria | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + 6 reduced adrenodoxin + 3 O2 | Bos taurus | first step in biosynthesis of all steroid hormones | pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
several ion exchange and heptyl-Sepharose hydrophobic chromatography | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Bos taurus | - |
corpus luteum | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
0°C on ice, purified, 20 mM potassium phosphate buffer, pH 7.4, 20% glycerol, v/v, 0.1 mM EDTA, 0.1 mM dithioerythritol, 0.01% Emulgen 911, 3 weeks, without substantial loss of activity | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + 6 reduced adrenodoxin + 3 O2 | first step in biosynthesis of all steroid hormones | Bos taurus | pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | - |
? | |
cholesterol + reduced adrenodoxin + O2 | - |
Bos taurus | pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 2,4,6,8 * 48000, SDS-PAGE, corpus luteum. x * 46000, x * 60000, x * 53000, x * 52000-53000 adrenal mitochondria | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adrenodoxin | - |
Bos taurus | |
heme | 0.78 heme group/unit of 48000 Da P-450, 1 mol of iron/44000 g | Bos taurus | |
NADPH | NADPH-linked adrenodoxin reductase | Bos taurus |