Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
D425V | 335fold reduced tyrosine hydroxylation/dopa formation activity, 120fold reduced reaction velocity with tyrosine, 3fold enhanced phenylalanine hydroxylation activity, active site mutant | Rattus norvegicus |
H323Y | enhanced Km for tyrosine, 4.5fold enhanced phenylalanine hydroxylation activity, active site mutant | Rattus norvegicus |
additional information | active site mutants of phenylalanine hydroxylase lead to highly increased tyrosine hydroxylation activity of the enzyme mutants | Rattus norvegicus |
Q310H | 4fold reduced tyrosine hydroxylation/dopa formation activity, slightly enhanced phenylalanine hydroxylation activity, active site mutant | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-values for tyrosine of mutant phenylalanine hydroxylase with tyrosine hydroxylation activity | Rattus norvegicus | |
0.00125 | - |
phenylalanine | recombinant mutant D425V | Rattus norvegicus | |
0.0077 | - |
phenylalanine | recombinant mutant H323Y | Rattus norvegicus | |
0.0088 | - |
phenylalanine | recombinant mutant Q310H | Rattus norvegicus | |
0.016 | - |
tyrosine | recombinant wild-type | Rattus norvegicus | |
0.045 | - |
tyrosine | recombinant mutant D425V | Rattus norvegicus | |
0.054 | - |
tyrosine | recombinant mutant Q310H | Rattus norvegicus | |
0.092 | - |
tyrosine | recombinant mutant H323Y | Rattus norvegicus | |
0.1 | - |
phenylalanine | recombinant wild-type | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P04177 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutants from Escherichia coli | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + tetrahydropteridine + 2 O2 | wild-type and mutants | Rattus norvegicus | 3,4-dihydroxy-L-phenylalanine + dihydropteridine + 2 H2O | - |
? | |
L-tyrosine + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | 3,4-dihydroxy-L-phenylalanine is identical with dopa | ? |
Synonyms | Comment | Organism |
---|---|---|
TH | - |
Rattus norvegicus |