Cloned (Comment) | Organism |
---|---|
medullary thyroid alpha-AE, type A, expression in chinese hamster ovary cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
H172A | mutant of copper ligand of peptidylglycine alpha-hydroxylating enzyme, reduced copper content below 0.3 Cu2+ per protein molecule | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | mutant H172A has reduced copper content: below 0.3 Cu2+ per protein molecule | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
bifunctional alpha-AE | - |
Purification (Comment) | Organism |
---|---|
mutant H172A of type A alpha-AE, recombinant from chinese hamster ovary cells | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[peptide]-glycine + 2 ascorbate + O2 = [peptide]-(2S)-2-hydroxyglycine + 2 monodehydroascorbate + H2O | structural analysis of mutant H172A, binding of ligands CO, Cu2+ | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
medullary thyroid carcinoma cell | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
monoiodo-alpha-N-acetyl-Tyr-Val-Gly + ascorbate + O2 | - |
Rattus norvegicus | monoiodo-alpha-N-acetyl-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O | - |
? | |
peptidylglycine + ascorbate + O2 | requirement for O2 | Rattus norvegicus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
peptidylglycine + ascorbate + O2 | COOH-terminal glycine | Rattus norvegicus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | - |
Rattus norvegicus |