Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dopamine + O2 | Agaricus bisporus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agaricus bisporus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Agaricus bisporus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dopamine + O2 | - |
Agaricus bisporus | ? | - |
? | |
dopamine + O2 | - |
Agaricus bisporus | dopamine quinone + H2O | - |
? | |
additional information | development of a facile fluorescent assay for TYR activity based on dopamine functionalized carbon quantum dots (CQDs-Dopa), method evaluation, overview. Dopamine (Dopa) is covalently bound to CQDs through a simple one-pot hydrothermal method, and the prepared CQDs-Dopa exhibits a fluorescence emission at 499 nm under exciting wavelength at 310 nm with a quantum yield of approximately 2.1%. When TYR is mixed with CODs-Dopa, the dopamine moiety in CQDs-Dopa conjugate is oxidized to O-dopaquinone, and an intra-particle photo-induced electron transfer process consequently occurs between CQDs and O-dopaquinone to quench the fluorescence of CQDs-Dopa. TYR activity can be determined based on the fluorescence quenching degree of CQDs-Dopa. The assay covers two broad linear ranges: 44.4-711.1 U/l and 711.1-2925.4 U/l with detection limit of 17.7 U/l. The proposed fluorescent assay is applied to TYR activity measurement in human serum samples and might be useful for TYR activity assays in clinical applications | Agaricus bisporus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mushroom tyrosine | - |
Agaricus bisporus |
tyr | - |
Agaricus bisporus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Agaricus bisporus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Agaricus bisporus |
General Information | Comment | Organism |
---|---|---|
physiological function | tyrosinase (TYR) is a key enzyme in melanin biosynthesis and its activity is an important biomarker for dermatological disorders, such as vitiligo, melanoma and actinic damages | Agaricus bisporus |