Cloned (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
expression in null mutant erg3 of Saccharomyces cerevisiae | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
G234A | no activity | Arabidopsis thaliana |
G234A | inactive mutant enzyme | Arabidopsis thaliana |
G234D | 2% of wild-type activity | Arabidopsis thaliana |
G234D | low activity | Arabidopsis thaliana |
H147E | no activity | Arabidopsis thaliana |
H147E | inactive mutant enzyme | Arabidopsis thaliana |
H147L | no activity | Arabidopsis thaliana |
H147L | inactive mutant enzyme | Arabidopsis thaliana |
H151E | no activity | Arabidopsis thaliana |
H151E | inactive mutant enzyme | Arabidopsis thaliana |
H151l | no activity | Arabidopsis thaliana |
H151l | inactive mutant enzyme | Arabidopsis thaliana |
H161E | no activity | Arabidopsis thaliana |
H161E | inactive mutant enzyme | Arabidopsis thaliana |
H161L | no activity | Arabidopsis thaliana |
H161L | inactive mutant enzyme | Arabidopsis thaliana |
H164L | no activity | Arabidopsis thaliana |
H164L | inactive mutant enzyme | Arabidopsis thaliana |
H165L | no activity | Arabidopsis thaliana |
H165L | inactive mutant enzyme | Arabidopsis thaliana |
H203L | reduced activity | Arabidopsis thaliana |
H203L | 13% of wild-type activity | Arabidopsis thaliana |
H222E | reduced activity | Arabidopsis thaliana |
H222E | 6% of wild-type activity | Arabidopsis thaliana |
H222L | no activity | Arabidopsis thaliana |
H222L | inactive mutant enzyme | Arabidopsis thaliana |
H238E | no activity | Arabidopsis thaliana |
H238E | inactive mutant enzyme | Arabidopsis thaliana |
H238L | no activity | Arabidopsis thaliana |
H238L | inactive mutant enzyme | Arabidopsis thaliana |
H241L | no activity | Arabidopsis thaliana |
H241L | inactive mutant enzyme | Arabidopsis thaliana |
H242L | no activity | Arabidopsis thaliana |
H242L | inactive mutant enzyme | Arabidopsis thaliana |
K115L | 2times higher activity than wild-type | Arabidopsis thaliana |
K115L | Km-value for 5alpha-cholest-7-en-3beta-ol is 2.8fold higher than that of wild-type activity | Arabidopsis thaliana |
P175A | wild-type activity | Arabidopsis thaliana |
P175A | as active as wild-type enzyme | Arabidopsis thaliana |
P175V | 4times higher activity than wild-type | Arabidopsis thaliana |
P175V | Km-value for 5alpha-cholest-7-en-3beta-ol is 2.7fold higher than that of wild-type activity | Arabidopsis thaliana |
P201A | 25% of wild-type activity | Arabidopsis thaliana |
P201A | 20% of wild-type activity | Arabidopsis thaliana |
T114I | wild-type activity | Arabidopsis thaliana |
T114I | | Km-value for 5alpha-cholest-7-en-3beta-ol is 7.5fold higher than that of wild-type activity | Arabidopsis thaliana |
T114S | 28times higher activity than wild-type | Arabidopsis thaliana |
T114S | Km-value for 5alpha-cholest-7-en-3beta-ol is 7.6fold higher than that of wild-type activity | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CN- | 0.003 mM, 50% inhibition of recombinant enzyme | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
5alpha-Cholest-7-en-3beta-ol | - |
Arabidopsis thaliana | |
0.032 | - |
5alpha-Cholest-7-en-3beta-ol | 30°C, wild-type enzyme | Arabidopsis thaliana | |
0.07 | - |
5alpha-Cholest-7-en-3beta-ol | P175a mutant enzyme | Arabidopsis thaliana | |
0.07 | - |
5alpha-Cholest-7-en-3beta-ol | 30°C, mutant enzyme P175A | Arabidopsis thaliana | |
0.086 | - |
5alpha-Cholest-7-en-3beta-ol | P175V mutant enzyme | Arabidopsis thaliana | |
0.086 | - |
5alpha-Cholest-7-en-3beta-ol | 30°C, mutant enzyme P175V | Arabidopsis thaliana | |
0.09 | - |
5alpha-Cholest-7-en-3beta-ol | K115L mutant enzyme | Arabidopsis thaliana | |
0.09 | - |
5alpha-Cholest-7-en-3beta-ol | 30°C, mutant enzyme K115L | Arabidopsis thaliana | |
0.24 | - |
5alpha-Cholest-7-en-3beta-ol | T114I mutant enzyme | Arabidopsis thaliana | |
0.24 | - |
5alpha-Cholest-7-en-3beta-ol | 30°C, mutant enzyme T114I | Arabidopsis thaliana | |
0.244 | - |
5alpha-Cholest-7-en-3beta-ol | T114S mutant enzyme | Arabidopsis thaliana | |
0.244 | - |
5alpha-Cholest-7-en-3beta-ol | 30°C, mutant enzyme T114S | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q39208 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-cholest-7-en-3beta-ol + ferrocytochrmoe b5 + H+ + O2 | NADH is more efficient than NADPH | Arabidopsis thaliana | cholesta-5,7-dien-3beta-ol + ferricytochrome b5 + H2O | - |
? | |
5alpha-cholest-7-en-3beta-ol + ferrocytochrome b5 + H+ + O2 | NADH is more efficient than NADPH | Arabidopsis thaliana | cholesta-5,7-dien-3beta-ol + ferricytochrome b5 + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5-DES | - |
Arabidopsis thaliana |
DELTA7-sterol 5-desaturase | - |
Arabidopsis thaliana |
DELTA7-sterol DELTA5-dehydrogenase | - |
Arabidopsis thaliana |
DELTA7-sterol-C5(6)-desaturase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | - |
Arabidopsis thaliana | |
NADH | NADH is more efficient than NADPH | Arabidopsis thaliana | |
NADPH | NADH is more efficient than NADPH | Arabidopsis thaliana |