Application | Comment | Organism |
---|---|---|
nutrition | potential of the engineered plastidial omega-3 desaturase from sesame to influence the profile of alpha-linolenic acid in transgenic tobacco plant by shifting the carbon flux from linoleic acid, the enzyme can be used in suitable genetic engineering strategy to increase the alpha-linolenic acid content in sesame and other vegetable oils | Sesamum indicum |
Cloned (Comment) | Organism |
---|---|
gene fad7, sequence comparisons, recombinant functional expression in Nicotiana tabacum cv. Jayanti seeds and leaves targeted to the endoplasmic reticulum, using the Agrobacterium tumefaciens strain EHA105 transfection method and the binary vector pCAMBIA1300 with CaMV35S promoter, quantitative real-time PCR expression analysis | Sesamum indicum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the predicted N-terminal plastidial signal peptide of fad7 gene is replaced by an endoplasmic reticulum signal peptide and an endoplasmic reticulum retention signal is placed at the C-terminal, enhancement of alpha-linolenic acid content in transgenic tobacco seeds by targeting the plastidial omega-3 fatty acid desaturase (fad7) gene from Sesamum indicum to the endoplasmic reticulum | Sesamum indicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | at the inner membrane of the chloroplast envelope, the gene possesses a chloroplast targeting signal sequence | Sesamum indicum | 9507 | - |
chloroplast envelope | - |
Sesamum indicum | 9941 | - |
thylakoid membrane | - |
Sesamum indicum | 42651 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | Sesamum indicum | - |
alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
alpha-linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | Sesamum indicum Var-9 | - |
alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sesamum indicum | P48620 | - |
- |
Sesamum indicum Var-9 | P48620 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | young | Sesamum indicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | - |
Sesamum indicum | alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
alpha-linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | - |
Sesamum indicum Var-9 | alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
additional information | FAD7 utilizes ferredoxin as the intermediate electron donor to act upon fatty acids esterified to galactolipids, sulfolipid, and phosphatidylglycerol | Sesamum indicum | ? | - |
? | |
additional information | FAD7 utilizes ferredoxin as the intermediate electron donor to act upon fatty acids esterified to galactolipids, sulfolipid, and phosphatidylglycerol | Sesamum indicum Var-9 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FAD7 | - |
Sesamum indicum |
fatty acid desaturase-7 | - |
Sesamum indicum |
plastidial omega-3 fatty acid desaturase | - |
Sesamum indicum |
Sefad7 | - |
Sesamum indicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | FAD7 utilizes ferredoxin as the intermediate electron donor to act upon fatty acids esterified to galactolipids, sulfolipid, and phosphatidylglycerol | Sesamum indicum |