BRENDA - Enzyme Database show
show all sequences of 1.14.19.65

Cloning and characterization of Cheilanthifoline and stylopine synthase genes from Chelidonium majus

Yahyazadeh, M.; Ratmoyo, P.; Bittner, F.; Sato, F.; Selmar, D.; Plant Cell Physiol. 58, 1421-1430 (2017)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
heterologously expressed in Saccharcomyces cerevisiae
Chelidonium majus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2
Chelidonium majus
the enzyme is involved in biosynthesis of stylopine
(S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Chelidonium majus
A0A240FWB3
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
expression is very similar in both roots and leaves, although the alkaloid accumulation patterns in these organs are quite different
Chelidonium majus
-
root
expression is very similar in both roots and leaves, although the alkaloid accumulation patterns in these organs are quite different
Chelidonium majus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2
high substrate specificity
746047
Chelidonium majus
(S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
(S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2
the enzyme is involved in biosynthesis of stylopine
746047
Chelidonium majus
(S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
heterologously expressed in Saccharcomyces cerevisiae
Chelidonium majus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2
Chelidonium majus
the enzyme is involved in biosynthesis of stylopine
(S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
expression is very similar in both roots and leaves, although the alkaloid accumulation patterns in these organs are quite different
Chelidonium majus
-
root
expression is very similar in both roots and leaves, although the alkaloid accumulation patterns in these organs are quite different
Chelidonium majus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2
high substrate specificity
746047
Chelidonium majus
(S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
(S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2
the enzyme is involved in biosynthesis of stylopine
746047
Chelidonium majus
(S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in biosynthesis of stylopine
Chelidonium majus
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in biosynthesis of stylopine
Chelidonium majus
Other publictions for EC 1.14.19.65
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746047
Yahyazadeh
-
Cloning and characterization ...
Chelidonium majus
Plant Cell Physiol.
58
1421-1430
2017
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1
1
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746447
Hori
Efficient microbial productio ...
Eschscholzia californica
Sci. Rep.
6
22201
2016
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2
1
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3
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1
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728446
Takemura
Molecular cloning and characte ...
Eschscholzia californica
Phytochemistry
91
100-108
2013
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1
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4
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711025
Diaz Chavez
Characterization of two methyl ...
Argemone mexicana
Arch. Biochem. Biophys.
507
186-193
2011
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1
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2
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1
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5
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1
1
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1
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1
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700725
Ikezawa
CYP719A subfamily of cytochrom ...
Eschscholzia californica
Plant Cell Rep.
28
123-133
2009
1
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1
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1
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6
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1
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1
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395597
Bauer
-
Two methylenedioxy bridge form ...
Eschscholzia californica
Phytochemistry
30
2953-2961
1991
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