BRENDA - Enzyme Database show
show all sequences of 1.14.19.68

New reactions in the protoberberine biosynthetic pathway

Rueffer, M.; Chem. Listy 87, 215-217 (1993)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
CO
inhibition can be partly reverted by blue light
Berberidaceae
CO
inhibition can be partly reverted by blue light
Ranunculaceae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
Berberidaceae
reaction in protoberberine biosynthetic pathway
(S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
Berberidaceae
-
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
Ranunculaceae
reaction in protoberberine biosynthetic pathway
(S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
Ranunculaceae
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Berberidaceae
-
-
-
Ranunculaceae
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
-
393237
Berberidaceae
(S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
393237
Berberidaceae
-
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
-
393237
Ranunculaceae
(S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
393237
Ranunculaceae
-
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
reaction in protoberberine biosynthetic pathway
393237
Berberidaceae
(S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
393237
Berberidaceae
-
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
reaction in protoberberine biosynthetic pathway
393237
Ranunculaceae
(S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
393237
Ranunculaceae
-
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
23% of the activity compared to NADPH
393237
Berberidaceae
?
-
-
-
-
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
23% of the activity compared to NADPH
393237
Ranunculaceae
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
cytochrome P-450 enzyme
Berberidaceae
cytochrome P450
cytochrome P-450 enzyme
Ranunculaceae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
cytochrome P-450 enzyme
Berberidaceae
cytochrome P450
cytochrome P-450 enzyme
Ranunculaceae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CO
inhibition can be partly reverted by blue light
Berberidaceae
CO
inhibition can be partly reverted by blue light
Ranunculaceae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
Berberidaceae
reaction in protoberberine biosynthetic pathway
(S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
Berberidaceae
-
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
Ranunculaceae
reaction in protoberberine biosynthetic pathway
(S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
Ranunculaceae
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
-
393237
Berberidaceae
(S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
393237
Berberidaceae
-
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
-
393237
Ranunculaceae
(S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
393237
Ranunculaceae
-
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
reaction in protoberberine biosynthetic pathway
393237
Berberidaceae
(S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
393237
Berberidaceae
-
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
reaction in protoberberine biosynthetic pathway
393237
Ranunculaceae
(S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
393237
Ranunculaceae
-
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
23% of the activity compared to NADPH
393237
Berberidaceae
?
-
-
-
-
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
23% of the activity compared to NADPH
393237
Ranunculaceae
?
-
-
-
-
Other publictions for EC 1.14.19.68
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743234
Galanie
Optimization of yeast-based p ...
Thalictrum flavum subsp. glaucum
Microb. Cell Fact.
14
144
2015
-
1
1
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
744778
He
-
Isolation and characterizatio ...
Coptis chinensis
Electron. J. Biotechnol.
18
376-380
2015
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
3
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
3
-
-
-
1
-
-
-
-
-
-
-
1
-
1
1
-
-
-
727532
Dang
Cloning and characterization o ...
Papaver somniferum
FEBS Lett.
588
198-204
2014
-
-
1
-
-
-
-
1
-
-
-
1
-
2
-
-
-
-
-
4
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
4
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
711025
Diaz Chavez
Characterization of two methyl ...
Argemone mexicana
Arch. Biochem. Biophys.
507
186-193
2011
-
-
1
-
-
-
-
1
-
-
1
-
-
5
-
-
-
-
-
4
-
-
1
-
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
4
-
-
1
-
1
1
-
-
1
1
-
-
-
1
1
-
-
-
676416
Samanani
Cell type-specific localizatio ...
Thalictrum flavum ssp. glaucum
Plant Cell
17
915-926
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393236
Rueffer
-
Canadine synthase from Thalict ...
Berberis aristata, Berberis crataegina, Berberis henryana, Berberis stolonifera, Berberis taliensis, Coptis japonica, Thalictrum glabrum, Thalictrum glaucum, Thalictrum macrocarpum, Thalictrum tuberosum
Phytochemistry
36
1219-1223
1994
-
-
-
-
-
-
11
2
1
-
-
1
-
10
-
-
-
-
-
2
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
11
-
2
1
-
-
1
-
-
-
-
-
2
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
393237
Rueffer
-
New reactions in the protoberb ...
Berberidaceae, Ranunculaceae
Chem. Listy
87
215-217
1993
-
-
-
-
-
-
2
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15195
Galneder
Alternative final steps in ber ...
Coptis japonica
Plant Cell Rep.
7
1-4
1988
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-