BRENDA - Enzyme Database show
show all sequences of 1.14.19.72

Next generation sequencing in predicting gene function in podophyllotoxin biosynthesis

Marques, J.V.; Kim, K.W.; Lee, C.; Costa, M.A.; May, G.D.; Crow, J.A.; Davin, L.B.; Lewis, N.G.; J. Biol. Chem. 288, 466-479 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene CYP719A23, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis
Sinopodophyllum hexandrum
gene CYP719A24, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis
Podophyllum peltatum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
steady-state Michaelis-Menten kinetics
Podophyllum peltatum
additional information
-
additional information
steady-state Michaelis-Menten kinetics
Sinopodophyllum hexandrum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2
Sinopodophyllum hexandrum
-
(-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2
Podophyllum peltatum
-
(-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
additional information
Sinopodophyllum hexandrum
the enzyme is capable of converting (-)-matairesinol into (-)-pluviatolide by catalyzing methylenedioxy bridge formation and does not act on other possible substrates tested, i.e. (+)-pinoresinol, (-)-pinoresinol, (-)-arctigenin, (-)-alpha-conidendrin, (+)-piperitol, (-)-piperitol, and (+)-phillygenin
?
-
-
-
additional information
Podophyllum peltatum
the enzyme is capable of converting (-)-matairesinol into (-)-pluviatolide by catalyzing methylenedioxy bridge formation and does not act on other possible substrates tested, i.e. (+)-pinoresinol, (-)-pinoresinol, (-)-arctigenin, (-)-alpha-conidendrin, (+)-piperitol, (-)-piperitol, and (+)-phillygenin
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Podophyllum peltatum
L7T720
gene CYP719A24
-
Sinopodophyllum hexandrum
L7T8H2
gene CYP719A23
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2
-
736439
Sinopodophyllum hexandrum
(-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2
-
736439
Podophyllum peltatum
(-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2
(-)-matairesinol is isolated from Forsythia intermedia
736439
Sinopodophyllum hexandrum
(-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O
product identification and quantification by 1H, 13C, and 13C,1H heteronuclear single quantum coherence NMR spectroscopic analyses
-
-
?
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2
(-)-matairesinol is isolated from Forsythia intermedia
736439
Podophyllum peltatum
(-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O
product identification and quantification by 1H, 13C, and 13C,1H heteronuclear single quantum coherence NMR spectroscopic analyses
-
-
?
additional information
the enzyme is capable of converting (-)-matairesinol into (-)-pluviatolide by catalyzing methylenedioxy bridge formation and does not act on other possible substrates tested, i.e. (+)-pinoresinol, (-)-pinoresinol, (-)-arctigenin, (-)-alpha-conidendrin, (+)-piperitol, (-)-piperitol, and (+)-phillygenin
736439
Sinopodophyllum hexandrum
?
-
-
-
-
additional information
the enzyme is capable of converting (-)-matairesinol into (-)-pluviatolide by catalyzing methylenedioxy bridge formation and does not act on other possible substrates tested, i.e. (+)-pinoresinol, (-)-pinoresinol, (-)-arctigenin, (-)-alpha-conidendrin, (+)-piperitol, (-)-piperitol, and (+)-phillygenin
736439
Podophyllum peltatum
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Podophyllum peltatum
25
-
assay at
Sinopodophyllum hexandrum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Podophyllum peltatum
7.5
-
assay at
Sinopodophyllum hexandrum
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Podophyllum peltatum
cytochrome P450
-
Sinopodophyllum hexandrum
Cloned(Commentary) (protein specific)
Commentary
Organism
gene CYP719A24, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis
Podophyllum peltatum
gene CYP719A23, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis
Sinopodophyllum hexandrum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Podophyllum peltatum
cytochrome P450
-
Sinopodophyllum hexandrum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
steady-state Michaelis-Menten kinetics
Podophyllum peltatum
additional information
-
additional information
steady-state Michaelis-Menten kinetics
Sinopodophyllum hexandrum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2
Sinopodophyllum hexandrum
-
(-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2
Podophyllum peltatum
-
(-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
additional information
Sinopodophyllum hexandrum
the enzyme is capable of converting (-)-matairesinol into (-)-pluviatolide by catalyzing methylenedioxy bridge formation and does not act on other possible substrates tested, i.e. (+)-pinoresinol, (-)-pinoresinol, (-)-arctigenin, (-)-alpha-conidendrin, (+)-piperitol, (-)-piperitol, and (+)-phillygenin
?
-
-
-
additional information
Podophyllum peltatum
the enzyme is capable of converting (-)-matairesinol into (-)-pluviatolide by catalyzing methylenedioxy bridge formation and does not act on other possible substrates tested, i.e. (+)-pinoresinol, (-)-pinoresinol, (-)-arctigenin, (-)-alpha-conidendrin, (+)-piperitol, (-)-piperitol, and (+)-phillygenin
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2
-
736439
Sinopodophyllum hexandrum
(-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2
-
736439
Podophyllum peltatum
(-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2
(-)-matairesinol is isolated from Forsythia intermedia
736439
Sinopodophyllum hexandrum
(-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O
product identification and quantification by 1H, 13C, and 13C,1H heteronuclear single quantum coherence NMR spectroscopic analyses
-
-
?
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2
(-)-matairesinol is isolated from Forsythia intermedia
736439
Podophyllum peltatum
(-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O
product identification and quantification by 1H, 13C, and 13C,1H heteronuclear single quantum coherence NMR spectroscopic analyses
-
-
?
additional information
the enzyme is capable of converting (-)-matairesinol into (-)-pluviatolide by catalyzing methylenedioxy bridge formation and does not act on other possible substrates tested, i.e. (+)-pinoresinol, (-)-pinoresinol, (-)-arctigenin, (-)-alpha-conidendrin, (+)-piperitol, (-)-piperitol, and (+)-phillygenin
736439
Sinopodophyllum hexandrum
?
-
-
-
-
additional information
the enzyme is capable of converting (-)-matairesinol into (-)-pluviatolide by catalyzing methylenedioxy bridge formation and does not act on other possible substrates tested, i.e. (+)-pinoresinol, (-)-pinoresinol, (-)-arctigenin, (-)-alpha-conidendrin, (+)-piperitol, (-)-piperitol, and (+)-phillygenin
736439
Podophyllum peltatum
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Podophyllum peltatum
25
-
assay at
Sinopodophyllum hexandrum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Podophyllum peltatum
7.5
-
assay at
Sinopodophyllum hexandrum
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in the podophyllotoxin biosynthesis, detailed pathway overview
Podophyllum peltatum
metabolism
the enzyme is involved in the podophyllotoxin biosynthesis, detailed pathway overview
Sinopodophyllum hexandrum
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in the podophyllotoxin biosynthesis, detailed pathway overview
Podophyllum peltatum
metabolism
the enzyme is involved in the podophyllotoxin biosynthesis, detailed pathway overview
Sinopodophyllum hexandrum
Other publictions for EC 1.14.19.72
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736439
Marques
Next generation sequencing in ...
Podophyllum peltatum, Sinopodophyllum hexandrum
J. Biol. Chem.
288
466-479
2013
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