Cloned (Comment) | Organism |
---|---|
astaxanthin yield in Saccharomyces cerevisiae is enhanced by combinational metabolic engineering and protein engineering targeting at the insufficient precursor supply and the weak downstream pathway capacity. Through introducing the positive GGPP synthase mutant CrtE03M together with overexpression of tHMG1, CrtI and CrtYB (from the cDNA of Xanthophyllomyces dendrorhous), the supply of the precursor beta-carotene is increased. Efficient conversion of beta-carotene to astaxanthin is achieved through increasing beta-carotene ketolase activity via directed evolution | Haematococcus lacustris |
Protein Variants | Comment | Organism |
---|---|---|
F298Y | improvement in canthaxanthin production | Haematococcus lacustris |
H165R | improvement in canthaxanthin production | Haematococcus lacustris |
H165R/F298Y | improvement in canthaxanthin production | Haematococcus lacustris |
H165R/V264D | improvement in canthaxanthin production | Haematococcus lacustris |
H165R/V264D/F298Y | mutant enzyme with 2.4fold improved activity | Haematococcus lacustris |
V264D | improvement in canthaxanthin production | Haematococcus lacustris |
V264D/F298Y | improvement in canthaxanthin production | Haematococcus lacustris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-carotene + 2 reduced acceptor + 2 O2 | Haematococcus lacustris | the enzyme is involved in the astaxanthin biosynthesis pathway | echinenone + 2 acceptor + 3 H2O | - |
? | |
echinenone + 2 reduced acceptor + 2 O2 | Haematococcus lacustris | the enzyme is involved in the astaxanthin biosynthesis pathway | canthaxanthin + 2 acceptor + 3 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haematococcus lacustris | E2DRF9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-carotene + 2 reduced acceptor + 2 O2 | the enzyme is involved in the astaxanthin biosynthesis pathway | Haematococcus lacustris | echinenone + 2 acceptor + 3 H2O | - |
? | |
echinenone + 2 reduced acceptor + 2 O2 | the enzyme is involved in the astaxanthin biosynthesis pathway | Haematococcus lacustris | canthaxanthin + 2 acceptor + 3 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-carotene ketolase | - |
Haematococcus lacustris |
OBKT | - |
Haematococcus lacustris |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the astaxanthin biosynthesis pathway | Haematococcus lacustris |