Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparison, His-tagged enzyme expression in Escherichia coli strains DH5alpha and M15 | Cryptosporidium parvum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | - |
Cryptosporidium parvum | |
additional information | no inhibition by NaN3 and KCN | Cryptosporidium parvum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | the enzyme contains an N-terminal mitochondrial targeting sequence | Cryptosporidium parvum | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | - |
Cryptosporidium parvum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
2 * 26000, recombinant enzyme, SDS-PAGE | Cryptosporidium parvum |
50000 | - |
recombinant enzyme, gel filtration | Cryptosporidium parvum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cryptosporidium parvum | Q5UVY7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strains DH5alpha and M15 by nickel affinity chromatography | Cryptosporidium parvum |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 26000, recombinant enzyme, SDS-PAGE | Cryptosporidium parvum |
Synonyms | Comment | Organism |
---|---|---|
Fe-SOD | - |
Cryptosporidium parvum |
iron-superoxide dismutase | - |
Cryptosporidium parvum |
mtSOD | - |
Cryptosporidium parvum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 70 | the recombinant purified enzyme is fairly stable at 4°C and 37°C, but is rapidly inactivated at 50°C and 70°C | Cryptosporidium parvum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Cryptosporidium parvum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | optimum pH-range | Cryptosporidium parvum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the recombinant purified enzyme is highly stable at neutral and alkaline pHs but is relatively unstable in acidic condition | Cryptosporidium parvum |
General Information | Comment | Organism |
---|---|---|
physiological function | SOD detoxifies the highly reactive superoxide anions to hydrogen peroxide and molecular oxygen | Cryptosporidium parvum |