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Literature summary for 1.15.1.1 extracted from
- Cloning, purification, and characterization of recombinant human extracellular superoxide dismutase in SF9 insect cells (2016), Mol. Cells, 39, 242-249 .
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | SOD3 has the potential as a therapeutic drug for various diseases | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene SOD3, cloning of EC-SOD and recombinant expression of wild-type and truncated mutant FLAG-tagged hEC-SOD enzymes in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method, both full length and truncated hEC-SOD proteins are enzymatically active | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of a catalytically active truncated (residues 19-240) mutant form of human EC-SOD (hEC-SOD) | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diethyldithiocarbamate | - |
Homo sapiens |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | required, enzyme-bound | Homo sapiens | |
| additional information | added Zn2+ and Cu2+ do not affect the enzyme activity or structure | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 superoxide + 2 H+ | Homo sapiens | - |
O2 + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P08294 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | recombinant hEC-SOD expressed in Sf9 insect cells is glycosylated at Asn107. The glycosylated form of hEC-SOD is soluble, whereas the unglycosylated form is not, but it can be solubilized by increasing the pH and ionic strength of the solution | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and truncated mutant FLAG-tagged hEC-SOD enzymes from Spodoptera frugiperda Sf9 cells by affinity chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 superoxide + 2 H+ | - |
Homo sapiens | O2 + H2O2 | - |
? | |
| additional information | enzyme activity detection by water-soluble tetrazolium (WST-1) assay. This assay is based on the detection of a water-soluble formazan dye that is formed upon reduction of water-soluble tetrazolium salt, WST-1, by the superoxide anion | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 33000, recombinant enzyme, SDS-PAGE | Homo sapiens |
| monomer | 1 * 33000, recombinant enzyme, SDS-PAGE | Homo sapiens |
| More | hEC-SOD is present both as a monomer (33 kDa) and a dimer (66 kDa), enzyme secondary structure determination by circular dichroism, recombinant hEC-SOD purified from Sf9 insect cells is mainly composed of beta-sheet structures | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EC-SOD | - |
Homo sapiens |
| extracellular superoxide dismutase | - |
Homo sapiens |
| hEC-SOD | - |
Homo sapiens |
| SOD3 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 9 | assay at | Homo sapiens |
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