Cloned (Comment) | Organism |
---|---|
gene LARa, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli, recombinant expression in transgenic Nicotianan tabacum and Arabidopsis thaliana via Agrobacterium tumefaciens strains EHA105 and C58C1?mediated transformation, quantitative real?time PCR enzyme expression analysis. In Arabidopsis thaliana, contents of both insoluble and soluble proanthocyanidins extracted from the seeds are reduced in the overexpressing CsLARs lines compared with wild-type, although CsLARs catalyze leucocyanidins conversion to catechin in vitro, no catechin is detected in any transgenic Arabidopsis thaliana lines. Also no proanthocyanidins are detected in the transgenic tobacco | Camellia sinensis |
gene LARb, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli, recombinant expression in transgenic Nicotianan tabacum and Arabidopsis thaliana via Agrobacterium tumefaciens strains EHA105 and C58C1-mediated transformation, quantitative real-time PCR enzyme expression analysis. In Arabidopsis thaliana, contents of both insoluble and soluble proanthocyanidins extracted from the seeds are reduced in the overexpressing CsLARs lines compared with wild-type, although CsLARs catalyze leucocyanidins conversion to catechin in vitro, no catechin is detected in any transgenic Arabidopsis thaliana lines. Also no proanthocyanidins are detected in the transgenic tobacco | Camellia sinensis |
gene LARc, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli, recombinant expression in transgenic Nicotianan tabacum and Arabidopsis thaliana via Agrobacterium tumefaciens strain s EHA105 and C58C1-mediated transformation, quantitative real-time PCR enzyme expression analysis. In Arabidopsis thaliana, contents of both insoluble and soluble proanthocyanidins extracted from the seeds are reduced in the overexpressing CsLARs lines compared with wild-type, although CsLARs catalyze leucocyanidins conversion to catechin in vitro, no catechin is detected in any transgenic Arabidopsis thaliana lines. Also no proanthocyanidins are detected in the transgenic tobacco | Camellia sinensis |
gene LARc, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli, recombinant expression in transgenic Nicotianan tabacum and Arabidopsis thaliana via Agrobacterium tumefaciens strain s EHA105 and C58C1?mediated transformation, quantitative real?time PCR enzyme expression analysis. In Arabidopsis thaliana, contents of both insoluble and soluble proanthocyanidins extracted from the seeds are reduced in the overexpressing CsLARs lines compared with wild-type, although CsLARs catalyze leucocyanidins conversion to catechin in vitro, no catechin is detected in any transgenic Arabidopsis thaliana lines. Also no proanthocyanidins are detected in the transgenic tobacco | Camellia sinensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+ | Camellia sinensis | - |
(2R,3S)-catechin + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Camellia sinensis | A0A286QXX3 | cv. Shuchazao, TRI2043, grown in an experimental tea field at Anhui agricultural university, Hefei, China (east longitude 117.27, north latitude 31.86) | - |
Camellia sinensis | A0A286QXY4 | cv. Shuchazao, TRI2043, grown in an experimental tea field at Anhui agricultural university, Hefei, China (east longitude 117.27, north latitude 31.86) | - |
Camellia sinensis | I1E425 | cv. Shuchazao, TRI2043, grown in an experimental tea field at Anhui agricultural university, Hefei, China (east longitude 117.27, north latitude 31.86) | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Camellia sinensis | - |
leaf bud | - |
Camellia sinensis | - |
additional information | accumulation of catechins is greater in the buds and younger leaves than in the mature leaves, stems and roots | Camellia sinensis | - |
root | - |
Camellia sinensis | - |
stem | young fine shoots | Camellia sinensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+ | - |
Camellia sinensis | (2R,3S)-catechin + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, about, sequence calculation | Camellia sinensis |
? | x * 36000, about, sequence calculation | Camellia sinensis |
? | x * 43000, about, sequence calculation | Camellia sinensis |
Synonyms | Comment | Organism |
---|---|---|
CsLAR | - |
Camellia sinensis |
LARa | - |
Camellia sinensis |
LarB | - |
Camellia sinensis |
LarC | - |
Camellia sinensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Camellia sinensis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Camellia sinensis | sequence calculation | - |
5.27 |
Camellia sinensis | sequence calculation | - |
5.34 |
Camellia sinensis | sequence calculation | - |
5.43 |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic analysis of the LAR family, overview | Camellia sinensis |
evolution | phylogenetic analysis of the LAR family, overview. The dicotyledonous LARs can be clustered into two subgroups, which are defined as cluster I and cluster II | Camellia sinensis |
malfunction | overexpression of CsLAR causes a decrease in the proanthocyanidins in transgenic plants | Camellia sinensis |
physiological function | the enzyme is required in the proanthocyanidin biosynthesis | Camellia sinensis |