Cloned (Comment) | Organism |
---|---|
high expression in Escherichia coli using the expression plasmid pFA002 | Ancylobacter aquaticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, 37% inhibition | Ancylobacter aquaticus | |
Hg2+ | 1 mM, complete inhibition | Ancylobacter aquaticus | |
NaN3 | 1 mM, complete inhibition | Ancylobacter aquaticus | |
NEM | 1 mM, 25.3% inhibition | Ancylobacter aquaticus | |
PCMB | 0.2 mM, complete inhibition | Ancylobacter aquaticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.057 | - |
NAD+ | pH 7.0, 30°C | Ancylobacter aquaticus | |
2.4 | - |
formate | pH 7.0, 30°C | Ancylobacter aquaticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
2 * 44000, SDS-PAGE | Ancylobacter aquaticus |
100000 | - |
gel filtration | Ancylobacter aquaticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ancylobacter aquaticus | Q845T0 | - |
- |
Ancylobacter aquaticus KNK607M | Q845T0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Ancylobacter aquaticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.47 | - |
- |
Ancylobacter aquaticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | - |
Ancylobacter aquaticus | CO2 + NADH + H+ | - |
? | |
formate + NAD+ | - |
Ancylobacter aquaticus KNK607M | CO2 + NADH + H+ | - |
? | |
formate + NADP+ | activity with NADP+ is 2.4% of the activity with NAD+ | Ancylobacter aquaticus | CO2 + NADPH | - |
? | |
formate + NADP+ | activity with NADP+ is 2.4% of the activity with NAD+ | Ancylobacter aquaticus KNK607M | CO2 + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 44000, SDS-PAGE | Ancylobacter aquaticus |
Synonyms | Comment | Organism |
---|---|---|
NAD-dependent formate dehydrogenase | - |
Ancylobacter aquaticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Ancylobacter aquaticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 60 | 35°C: about 50% of maximal activity, 60°C: about 45% of maximal activity | Ancylobacter aquaticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 7.0, 10 min, stable at or lower | Ancylobacter aquaticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
- |
Ancylobacter aquaticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10.5 | pH 5.0: about 60% of maximal activity, pH 5.3: about 80% of maximal activity, pH 9.6: about 80% of maximal activity, pH 10.5: about 65% of maximal activity | Ancylobacter aquaticus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
30°C, 20 h, about 50% of initial activity | Ancylobacter aquaticus |
7 | - |
30°C, 20 h, most stable at | Ancylobacter aquaticus |
8 | - |
30°C, 20 h, about 25% of initial activity | Ancylobacter aquaticus |
8.5 | - |
30°C, 20 h, about 40% of initial activity | Ancylobacter aquaticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Ancylobacter aquaticus | |
NADP+ | activity is 2.4% of the activity with NAD+ | Ancylobacter aquaticus |