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Literature summary for 1.17.1.9 extracted from

  • Choe, H.; Ha, J.M.; Joo, J.C.; Kim, H.; Yoon, H.J.; Kim, S.; Son, S.H.; Gengan, R.M.; Jeon, S.T.; Chang, R.; Jung, K.D.; Kim, Y.H.; Lee, H.H.
    Structural insights into the efficient CO2-reducing activity of an NAD-dependent formate dehydrogenase from Thiobacillus sp. KNK65MA (2015), Acta Crystallogr. Sect. D, 71, 313-323.
    View publication on PubMed
Search for more literature for 1.17.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thiobacillus sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M HEPES pH 7.0, 25% (w/v) polyethylene glycol 6000, 1.2 M lithium chloride Thiobacillus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formate + NAD+ Thiobacillus sp.
-
 CO2 + NADH + H+
-
 ?
formate + NAD+ Thiobacillus sp. KNK65MA
-
 CO2 + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Thiobacillus sp. Q76EB7
-
-
Thiobacillus sp. KNK65MA Q76EB7
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography and Superdex 200 gel filtration Thiobacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 Thiobacillus sp. CO2 + NADH + H+
-
 ?
formate + NAD+
-
 Thiobacillus sp. KNK65MA CO2 + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
NAD-dependent FDH
-
 Thiobacillus sp.
NAD-dependent formate dehydrogenase
-
 Thiobacillus sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Thiobacillus sp.