Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | not inactivated by molecular oxygen | Rhodobacter capsulatus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rhodobacter capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.173 | - |
NAD+ | at pH 9.0 and 30°C | Rhodobacter capsulatus | |
0.277 | - |
formate | with 2,6-dichlorophenolindophenol as cosubstrate, at pH 9.0 and 30°C | Rhodobacter capsulatus | |
0.278 | - |
formate | with ferricyanide as cosubstrate, at pH 9.0 and 30°C | Rhodobacter capsulatus | |
0.281 | - |
formate | with NAD+ as cosubstrate, at pH 9.0 and 30°C | Rhodobacter capsulatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rhodobacter capsulatus | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
- |
Rhodobacter capsulatus |
50000 | - |
- |
Rhodobacter capsulatus |
100000 | - |
- |
Rhodobacter capsulatus |
340000 | - |
gel filtration | Rhodobacter capsulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | Rhodobacter capsulatus | - |
CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + 2,6-dichlorophenolindophenol | - |
Rhodobacter capsulatus | CO2 + reduced 2,6-dichlorophenolindophenol | - |
? | |
formate + ferricyanide | - |
Rhodobacter capsulatus | CO2 + ferrocyanide | - |
? | |
formate + NAD+ | - |
Rhodobacter capsulatus | CO2 + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | 2 * 100000 + 2 * 50000 + 2 * 15000, SDS-PAGE | Rhodobacter capsulatus |
Synonyms | Comment | Organism |
---|---|---|
FDH | - |
Rhodobacter capsulatus |
NAD+-dependent formate dehydrogenase | - |
Rhodobacter capsulatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Rhodobacter capsulatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
CO2 | with NADH as cosubstrate, at pH 9.0 and 30°C | Rhodobacter capsulatus | |
32 | - |
formate | with 2,6-dichlorophenolindophenol as cosubstrate, at pH 9.0 and 30°C | Rhodobacter capsulatus | |
36.5 | - |
formate | with NAD+ as cosubstrate, at pH 9.0 and 30°C | Rhodobacter capsulatus | |
39.7 | - |
formate | with ferricyanide as cosubstrate, at pH 9.0 and 30°C | Rhodobacter capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Rhodobacter capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
bis-molybdopterin guanine dinucleotide | - |
Rhodobacter capsulatus | |
FMN | - |
Rhodobacter capsulatus | |
NAD+ | - |
Rhodobacter capsulatus | |
[2Fe-2S]-center | - |
Rhodobacter capsulatus | |
[4Fe-4S]-center | - |
Rhodobacter capsulatus |