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Literature summary for 1.17.1.9 extracted from
- Molybdenum-containing membrane-bound formate dehydrogenase isolated from Citrobacter sp. S-77 having high stability against oxygen, pH, and temperature (2014), J. Biosci. Bioeng., 118, 386-391.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.44 | - |
formate | at pH 7.0 and 30°C | Citrobacter sp. |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Citrobacter sp. | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Molybdenum | contains molybdenum | Citrobacter sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
- |
Citrobacter sp. |
| 95000 | - |
- |
Citrobacter sp. |
| 150000 | - |
gel filtration | Citrobacter sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formate + NAD+ | Citrobacter sp. | - |
CO2 + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrobacter sp. | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| the oxidized enzyme can be restored up to 80% of its original specific activity, even after the enzyme is exposed to air for 6 h at 4°C | Citrobacter sp. |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| hydroxyapatite column chromatography, Q Sepharose column chromatography, Resource 15 Q column chromatography, and Superdex 200 gel filtration | Citrobacter sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formate + benzyl viologen | - |
Citrobacter sp. | CO2 + reduced benzyl viologen | - |
? | |
| formate + NAD+ | - |
Citrobacter sp. | CO2 + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 95000 + x * 32000, SDS-PAGE | Citrobacter sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FDH | - |
Citrobacter sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
the enzyme has a half-life of activity of approximately 20 min at 70°C under anaerobic conditions | Citrobacter sp. |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 76 | - |
formate | at pH 7.0 and 30°C | Citrobacter sp. | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Citrobacter sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
the enzyme retains more than 70% of the initial activity after 3 h incubation at pH 3.0 | Citrobacter sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| molybdenum cofactor | - |
Citrobacter sp. | |
| NAD+ | - |
Citrobacter sp. | |
| [4Fe-4S]-center | - |
Citrobacter sp. | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 173 | - |
formate | at pH 7.0 and 30°C | Citrobacter sp. | |
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