Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
The enzyme complex is resolved into its constituent proteins by means of gel filtration on Sepharose CL-6B in the presence of 2 M-KI, followed by chromatography on hydroxyapatite in the presence of 8 M urea. These harsh conditions are necessary to cause suitable dissociation of the enzyme complex | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.4 | - |
30°C, pH not specified in the publication | Geobacillus stearothermophilus |
Subunits | Comment | Organism |
---|---|---|
multimer | x * 57000, x * 54000, x * 42000 and x * 36000, respectively, SDS-PAGE. The empirical unit must be repeated at least 50 times to make up the assembled complex | Geobacillus stearothermophilus |