Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15 cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
in complex with S-methyl-l-cysteine sulfoxide and sulfate, sitting drop vapor diffusion method, using 1.6 M ammonium sulfate and 100 mM citric acid pH 5.0 | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-4-aspartyl phosphate + NADPH + H+ | Mycobacterium tuberculosis | - |
L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? | |
L-4-aspartyl phosphate + NADPH + H+ | Mycobacterium tuberculosis H37Rv | - |
L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WNX5 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WNX5 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-4-aspartyl phosphate + NADPH + H+ | - |
Mycobacterium tuberculosis | L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? | |
L-4-aspartyl phosphate + NADPH + H+ | - |
Mycobacterium tuberculosis H37Rv | L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ASADH | - |
Mycobacterium tuberculosis |
Asd | - |
Mycobacterium tuberculosis |
Rv3708c | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme lies at the first branch point in the biosynthetic pathway of important amino acids including lysine and methionine and the cell-wall component diaminopimelate | Mycobacterium tuberculosis |