Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | - |
Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
monomer, determined by SDS-PAGE | Oryctolagus cuniculus |
144000 | - |
tetramer, calculated | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Oryctolagus cuniculus | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
GAPDH is subjected to gel filtration on a Sephadex G-100 column | Oryctolagus cuniculus |
Renatured (Comment) | Organism |
---|---|
GroEL-assisted reactivation after denaturation of GAPDH in the presence of guanidine hydrochloride | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Oryctolagus cuniculus | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Oryctolagus cuniculus |
Synonyms | Comment | Organism |
---|---|---|
GAPDH | - |
Oryctolagus cuniculus |
glyceraldehyde-3-phosphate dehydrogenase | - |
Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
activity assay | Oryctolagus cuniculus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
100% thermoinactivation of GAPDH in the absence and in the presence of GroEL after 80 and 60 min, respectively, at 45°C | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.9 | - |
activity assay | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Oryctolagus cuniculus |