Cloned (Comment) | Organism |
---|---|
recombinant expression of untagged enzyme in Escherichia coli strain Rosetta 2 (DE3) | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Homo sapiens | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P04406 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant untagged enzyme partially from Escherichia coli strain Rosetta 2 (DE3) by ammonium sulfate fractionation and precipitation in cyrstals, followed by desalting gel filtration, to homogeneity, method evaluation | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
after 6 months of storage, the specific activity of hGAPDH decreases to 10-15% of the original value due to the reversible oxidation of the enzyme. Reactivation of the enzyme by dissolving in 10 mM potassium phosphate, pH 7.4, and 5 mM DTT, to 1 mg/ml concentration, 2-4 h of incubation at 20-25°C, the specific activity of hGAPDH increases 5-7-fold. Alternatively, the enzyme can be dialyzed against required buffer solution containing 5 mM DTT | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
neuroblastoma cell | - |
Homo sapiens | - |
SH-SY5Y cell | - |
Homo sapiens | - |
Storage Stability | Organism |
---|---|
after 6 months of storage, the specific activity of hGAPDH decreases to 10-15% of the original value due to the reversible oxidation of the enzyme. Reactivation of the enzyme by dissolving in 10 mM potassium phosphate, pH 7.4, and 5 mM DTT, to 1 mg/ml concentration, 2-4 h of incubation at 20-25°C, the specific activity of hGAPDH increases 5-7-fold. Alternatively, the enzyme can be dialyzed against required buffer solution containing 5 mM DTT | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Homo sapiens | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | human GAPDH identified by peptide mass fingerprinting and mass spectrometric analysis | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
GAPDH | - |
Homo sapiens |
glyceraldehyde-3-phosphate dehydrogenase | - |
Homo sapiens |
hGAPDH | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | sequence calculation | - |
8.3 |
General Information | Comment | Organism |
---|---|---|
additional information | denatured GAPDH, in contrast to the native enzyme, interacts with the bacterial chaperonin GroEL and beta-amyloid peptide 1-42 | Homo sapiens |
physiological function | glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a glycolytic enzyme, whose main role is to provide energy for different cellular functions. Also the enzyme appears to be involved in numerous cell processes that have no relation to glycolysis | Homo sapiens |