Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rattus norvegicus |
functional expression of wild-type and chimeric mutant enzymes in Escherichia coli strain BL21(DE3) | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of recombinant chimeric enzymes exchanging parts of the enzyme by sequences of the phenobarbital-induced aldehyde dehydrogenase from rat, and vice versa, the chimeric enzymes show altered substrate specificity towards 9-cis- or all-trans-retinal, overview | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, recombinant wild-type and chimeric mutant enzymes | Rattus norvegicus | |
0.0087 | - |
9-cis-retinal | 25°C | Rattus norvegicus | |
0.0087 | - |
9-cis-retinal | recombinant wild-type enzyme, pH 7.2, 25°C | Rattus norvegicus | |
0.01 | - |
all-trans-retinal | 25°C | Rattus norvegicus | |
0.01 | - |
all-trans-retinal | recombinant wild-type enzyme, pH 7.2, 25°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, recombinant enzyme, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
retinal + NAD+ + H2O | Rattus norvegicus | - |
retinoate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Rattus norvegicus | - |
class I enzyme | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
retinal + NAD+ + H2O = retinoate + NADH + H+ | catalytic active nucleophil Cys302, not responsible for substrate specificity | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
9-cis-retinal + NAD+ + H2O | - |
Rattus norvegicus | 9-cis-retinoate + NADH | - |
? | |
9-cis-retinal + NAD+ + H2O | poortly active with | Rattus norvegicus | 9-cis-retinoate + NADH | - |
? | |
all-trans-retinal + NAD+ + H2O | - |
Rattus norvegicus | all-trans-retinoate + NADH | - |
? | |
propanal + NAD+ + H2O | - |
Rattus norvegicus | propionate + NADH | - |
? | |
retinal + NAD+ + H2O | - |
Rattus norvegicus | retinoate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, recombinant enzyme, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
PB-RALDH | - |
Rattus norvegicus |
phenobarbital-induced aldehyde dehydrogenase | - |
Rattus norvegicus |
RALDH | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
about, assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rattus norvegicus |