Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15 cells | Selaginella moellendorffii |
Protein Variants | Comment | Organism |
---|---|---|
S123T | the mutant shows reduced activity compared to the wild type enzyme | Selaginella moellendorffii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02759 | - |
feruloyl-CoA | isoform CCR1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
0.05092 | - |
sinapoyl-CoA | isoform CCR2-1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
0.05581 | - |
4-coumaroyl-CoA | isoform CCR1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
0.11608 | - |
4-coumaroyl-CoA | isoform CCR2-1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
0.13699 | - |
feruloyl-CoA | isoform CCR2-1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
0.15233 | - |
caffeoyl-CoA | isoform CCR2-1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
0.28301 | - |
caffeoyl-CoA | isoform CCR1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
0.56836 | - |
sinapoyl-CoA | isoform CCR1, pH and temperature not specified in the publication | Selaginella moellendorffii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Selaginella moellendorffii | - |
- |
- |
Selaginella moellendorffii | A0A1V0HSA2 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Selaginella moellendorffii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + NADPH + H+ | - |
Selaginella moellendorffii | 4-coumaraldehyde + CoA + NADP+ | - |
? | |
caffeoyl-CoA + NADPH + H+ | - |
Selaginella moellendorffii | caffeylaldehyde + NADP+ | - |
? | |
feruloyl-CoA + NADPH + H+ | - |
Selaginella moellendorffii | coniferaldehyde + CoA + NADP+ | - |
? | |
feruloyl-CoA + NADPH + H+ | preferred substrate of isoform CCR1 | Selaginella moellendorffii | coniferaldehyde + CoA + NADP+ | - |
? | |
sinapoyl-CoA + NADPH + H+ | - |
Selaginella moellendorffii | sinapaldehyde + CoA + NADP+ | - |
? | |
sinapoyl-CoA + NADPH + H+ | preferred substrate of isoform CCR2-1 | Selaginella moellendorffii | sinapaldehyde + CoA + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36000, SDS-PAGE | Selaginella moellendorffii |
Synonyms | Comment | Organism |
---|---|---|
CCR1 | isoform | Selaginella moellendorffii |
CCR2-1 | isoform | Selaginella moellendorffii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Selaginella moellendorffii |
50 | - |
- |
Selaginella moellendorffii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 60 | the enzyme activity is optimal at 30°C, with a steep decrease when the reaction is performed at 40°C. Isoform CCR1 exhibits relatively high activity at low temperature | Selaginella moellendorffii |
10 | 60 | the enzyme shows approximately 50% activity at 60°C, approximately 5.3% activity at 20°C and no activity at 10°C | Selaginella moellendorffii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
4-coumaroyl-CoA | mutant S123T of isoform CCR1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
0.052 | - |
sinapoyl-CoA | mutant S123T of isoform CCR1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
0.093 | - |
caffeoyl-CoA | mutant S123T of isoform CCR1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
0.136 | - |
feruloyl-CoA | mutant S123T of isoform CCR1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
0.232 | - |
4-coumaroyl-CoA | isoform CCR2-1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
0.24 | - |
feruloyl-CoA | isoform CCR2-1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
0.261 | - |
sinapoyl-CoA | isoform CCR2-1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
0.289 | - |
feruloyl-CoA | isoform CCR1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
0.324 | - |
4-coumaroyl-CoA | isoform CCR1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
0.326 | - |
caffeoyl-CoA | isoform CCR2-1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
0.559 | - |
caffeoyl-CoA | isoform CCR1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
0.829 | - |
sinapoyl-CoA | isoform CCR1, pH and temperature not specified in the publication | Selaginella moellendorffii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Selaginella moellendorffii |
6.5 | 7 | - |
Selaginella moellendorffii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Selaginella moellendorffii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
sinapoyl-CoA | isoform CCR1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
1.8 | - |
feruloyl-CoA | isoform CCR2-1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
2 | - |
caffeoyl-CoA | isoform CCR1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
2 | - |
4-coumaroyl-CoA | isoform CCR2-1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
2.2 | - |
caffeoyl-CoA | isoform CCR2-1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
4.3 | - |
4-coumaroyl-CoA | isoform CCR1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
5.2 | - |
sinapoyl-CoA | isoform CCR2-1, pH and temperature not specified in the publication | Selaginella moellendorffii | |
10.5 | - |
feruloyl-CoA | isoform CCR1, pH and temperature not specified in the publication | Selaginella moellendorffii |