BRENDA - Enzyme Database
show all sequences of 1.2.1.58

Phenylglyoxylate:NAD+ oxidoreductase (CoA benzoylating), a new enzyme of anaerobic phenylalanine metabolism in the denitrifying bacterium Azoarcus evansii

Hirsch, W.; Schägger, H.; Fuchs, G.; Eur. J. Biochem. 251, 907-915 (1998)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.045
-
phenylglyoxylate
-
Aromatoleum evansii
0.055
-
CoA
-
Aromatoleum evansii
0.074
-
NAD+
-
Aromatoleum evansii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
iron-sulfur protein, contains 35 mol Fe and 36 mol acid-labile sulfur per mol of enzyme
Aromatoleum evansii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
11500
-
2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase
Aromatoleum evansii
24000
-
2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase
Aromatoleum evansii
43000
-
2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase
Aromatoleum evansii
48000
-
2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase
Aromatoleum evansii
50000
-
2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase
Aromatoleum evansii
370000
-
gel filtration
Aromatoleum evansii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
phenylglyoxylate + NAD+ + CoA-SH
Aromatoleum evansii
enzyme activity is induced during anaerobic growth with Phe, phenylacetate and phenylglyoxylate, but not with benzoate
benzoyl-S-CoA + CO2 + NADH
-
-
?
phenylglyoxylate + NAD+ + CoA-SH
Aromatoleum evansii
enzyme of anaerobic Phe metabolism
benzoyl-S-CoA + CO2 + NADH
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Aromatoleum evansii
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Aromatoleum evansii
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.1
-
NAD+-dependent reaction
Aromatoleum evansii
7.4
-
benzyl viologen-dependent reaction
Aromatoleum evansii
Storage Stability
Storage Stability
Organism
6°C, 1 mM phenylglyoxal, half-life: 2 days
Aromatoleum evansii
6°C, 10% glycerol, half-life: 4 days
Aromatoleum evansii
6°C, without glycerol or substrate, half-life: 10 h
Aromatoleum evansii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-oxoisovalerate + NAD+ + CoA-SH
at 15% of the activity relative to phenylglyoxylate
11535
Aromatoleum evansii
?
-
-
-
?
additional information
enzyme catalyzes a NAD(H):viologen dye transhydrogenation
11535
Aromatoleum evansii
?
-
-
-
?
phenylglyoxylate + benzyl viologen + CoA-SH
-
11535
Aromatoleum evansii
benzoyl-S-CoA + CO2 + reduced benzyl viologen
-
11535
Aromatoleum evansii
?
phenylglyoxylate + FAD + CoA-SH
-
11535
Aromatoleum evansii
benzoyl-S-CoA + CO2 + FADH2
-
-
-
?
phenylglyoxylate + FMN + CoA-SH
-
11535
Aromatoleum evansii
benzoyl-S-CoA + CO2 + FMNH
-
-
-
?
phenylglyoxylate + methyl viologen + CoA-SH
-
11535
Aromatoleum evansii
benzoyl-S-CoA + CO2 + reduced methyl viologen
-
-
-
?
phenylglyoxylate + NAD+ + CoA-SH
reaction is strictly dependent on CoA
11535
Aromatoleum evansii
benzoyl-S-CoA + CO2 + NADH
-
11535
Aromatoleum evansii
?
phenylglyoxylate + NAD+ + CoA-SH
enzyme activity is induced during anaerobic growth with Phe, phenylacetate and phenylglyoxylate, but not with benzoate
11535
Aromatoleum evansii
benzoyl-S-CoA + CO2 + NADH
-
-
-
?
phenylglyoxylate + NAD+ + CoA-SH
enzyme of anaerobic Phe metabolism
11535
Aromatoleum evansii
benzoyl-S-CoA + CO2 + NADH
-
-
-
?
Subunits
Subunits
Commentary
Organism
decamer
2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase
Aromatoleum evansii
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
46
-
benzyl viologen
-
Aromatoleum evansii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Aromatoleum evansii
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
contains 1.1 mol FAD per mol of enzyme
Aromatoleum evansii
thiamine diphosphate
reaction is strictly dependent on
Aromatoleum evansii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
contains 1.1 mol FAD per mol of enzyme
Aromatoleum evansii
thiamine diphosphate
reaction is strictly dependent on
Aromatoleum evansii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.045
-
phenylglyoxylate
-
Aromatoleum evansii
0.055
-
CoA
-
Aromatoleum evansii
0.074
-
NAD+
-
Aromatoleum evansii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
iron-sulfur protein, contains 35 mol Fe and 36 mol acid-labile sulfur per mol of enzyme
Aromatoleum evansii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
11500
-
2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase
Aromatoleum evansii
24000
-
2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase
Aromatoleum evansii
43000
-
2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase
Aromatoleum evansii
48000
-
2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase
Aromatoleum evansii
50000
-
2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase
Aromatoleum evansii
370000
-
gel filtration
Aromatoleum evansii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
phenylglyoxylate + NAD+ + CoA-SH
Aromatoleum evansii
enzyme activity is induced during anaerobic growth with Phe, phenylacetate and phenylglyoxylate, but not with benzoate
benzoyl-S-CoA + CO2 + NADH
-
-
?
phenylglyoxylate + NAD+ + CoA-SH
Aromatoleum evansii
enzyme of anaerobic Phe metabolism
benzoyl-S-CoA + CO2 + NADH
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Aromatoleum evansii
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.1
-
NAD+-dependent reaction
Aromatoleum evansii
7.4
-
benzyl viologen-dependent reaction
Aromatoleum evansii
Storage Stability (protein specific)
Storage Stability
Organism
6°C, 1 mM phenylglyoxal, half-life: 2 days
Aromatoleum evansii
6°C, 10% glycerol, half-life: 4 days
Aromatoleum evansii
6°C, without glycerol or substrate, half-life: 10 h
Aromatoleum evansii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-oxoisovalerate + NAD+ + CoA-SH
at 15% of the activity relative to phenylglyoxylate
11535
Aromatoleum evansii
?
-
-
-
?
additional information
enzyme catalyzes a NAD(H):viologen dye transhydrogenation
11535
Aromatoleum evansii
?
-
-
-
?
phenylglyoxylate + benzyl viologen + CoA-SH
-
11535
Aromatoleum evansii
benzoyl-S-CoA + CO2 + reduced benzyl viologen
-
11535
Aromatoleum evansii
?
phenylglyoxylate + FAD + CoA-SH
-
11535
Aromatoleum evansii
benzoyl-S-CoA + CO2 + FADH2
-
-
-
?
phenylglyoxylate + FMN + CoA-SH
-
11535
Aromatoleum evansii
benzoyl-S-CoA + CO2 + FMNH
-
-
-
?
phenylglyoxylate + methyl viologen + CoA-SH
-
11535
Aromatoleum evansii
benzoyl-S-CoA + CO2 + reduced methyl viologen
-
-
-
?
phenylglyoxylate + NAD+ + CoA-SH
reaction is strictly dependent on CoA
11535
Aromatoleum evansii
benzoyl-S-CoA + CO2 + NADH
-
11535
Aromatoleum evansii
?
phenylglyoxylate + NAD+ + CoA-SH
enzyme activity is induced during anaerobic growth with Phe, phenylacetate and phenylglyoxylate, but not with benzoate
11535
Aromatoleum evansii
benzoyl-S-CoA + CO2 + NADH
-
-
-
?
phenylglyoxylate + NAD+ + CoA-SH
enzyme of anaerobic Phe metabolism
11535
Aromatoleum evansii
benzoyl-S-CoA + CO2 + NADH
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
decamer
2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase
Aromatoleum evansii
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
46
-
benzyl viologen
-
Aromatoleum evansii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Aromatoleum evansii
Other publictions for EC 1.2.1.58
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
11535
Hirsch
Phenylglyoxylate:NAD+ oxidored ...
Aromatoleum evansii
Eur. J. Biochem.
251
907-915
1998
-
-
-
-
-
-
-
3
-
1
6
2
-
5
-
-
1
-
-
-
2
3
9
1
-
-
-
-
1
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
3
-
1
6
2
-
-
-
1
-
-
2
3
9
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
718
Schneider
Anaerobic metabolism of L-phen ...
Thauera aromatica
Arch. Microbiol.
168
310-320
1997
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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