KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
phenylglyoxylate | - |
Aromatoleum evansii | |
0.055 | - |
CoA | - |
Aromatoleum evansii | |
0.074 | - |
NAD+ | - |
Aromatoleum evansii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | iron-sulfur protein, contains 35 mol Fe and 36 mol acid-labile sulfur per mol of enzyme | Aromatoleum evansii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
11500 | - |
2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase | Aromatoleum evansii |
24000 | - |
2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase | Aromatoleum evansii |
43000 | - |
2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase | Aromatoleum evansii |
48000 | - |
2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase | Aromatoleum evansii |
50000 | - |
2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase | Aromatoleum evansii |
370000 | - |
gel filtration | Aromatoleum evansii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenylglyoxylate + NAD+ + CoA-SH | Aromatoleum evansii | enzyme activity is induced during anaerobic growth with Phe, phenylacetate and phenylglyoxylate, but not with benzoate | benzoyl-S-CoA + CO2 + NADH | - |
? | |
phenylglyoxylate + NAD+ + CoA-SH | Aromatoleum evansii | enzyme of anaerobic Phe metabolism | benzoyl-S-CoA + CO2 + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aromatoleum evansii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aromatoleum evansii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.1 | - |
NAD+-dependent reaction | Aromatoleum evansii |
7.4 | - |
benzyl viologen-dependent reaction | Aromatoleum evansii |
Storage Stability | Organism |
---|---|
6°C, 1 mM phenylglyoxal, half-life: 2 days | Aromatoleum evansii |
6°C, 10% glycerol, half-life: 4 days | Aromatoleum evansii |
6°C, without glycerol or substrate, half-life: 10 h | Aromatoleum evansii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoisovalerate + NAD+ + CoA-SH | at 15% of the activity relative to phenylglyoxylate | Aromatoleum evansii | ? | - |
? | |
additional information | enzyme catalyzes a NAD(H):viologen dye transhydrogenation | Aromatoleum evansii | ? | - |
? | |
phenylglyoxylate + benzyl viologen + CoA-SH | - |
Aromatoleum evansii | benzoyl-S-CoA + CO2 + reduced benzyl viologen | - |
? | |
phenylglyoxylate + FAD + CoA-SH | - |
Aromatoleum evansii | benzoyl-S-CoA + CO2 + FADH2 | - |
? | |
phenylglyoxylate + FMN + CoA-SH | - |
Aromatoleum evansii | benzoyl-S-CoA + CO2 + FMNH | - |
? | |
phenylglyoxylate + methyl viologen + CoA-SH | - |
Aromatoleum evansii | benzoyl-S-CoA + CO2 + reduced methyl viologen | - |
? | |
phenylglyoxylate + NAD+ + CoA-SH | reaction is strictly dependent on CoA | Aromatoleum evansii | benzoyl-S-CoA + CO2 + NADH | - |
? | |
phenylglyoxylate + NAD+ + CoA-SH | enzyme activity is induced during anaerobic growth with Phe, phenylacetate and phenylglyoxylate, but not with benzoate | Aromatoleum evansii | benzoyl-S-CoA + CO2 + NADH | - |
? | |
phenylglyoxylate + NAD+ + CoA-SH | enzyme of anaerobic Phe metabolism | Aromatoleum evansii | benzoyl-S-CoA + CO2 + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | 2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase | Aromatoleum evansii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
46 | - |
benzyl viologen | - |
Aromatoleum evansii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Aromatoleum evansii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains 1.1 mol FAD per mol of enzyme | Aromatoleum evansii | |
thiamine diphosphate | reaction is strictly dependent on | Aromatoleum evansii |